Evidence map›Paper›PMID 36409550›Full record

ArticleBiology open2022

Correlative light and electron microscopy reveals fork-shaped structures at actin entry sites of focal adhesions.

Karin Legerstee, Jason Sueters, Tsion E Abraham, Johan A Slotman, Gert-Jan Kremers, Jacob P Hoogenboom, Adriaan B Houtsmuller

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Karin LegersteeErasmus Medical Centre Rotterdam, Department of Pathology, Optical Imaging Centre, 3000 CA, Rotterdam, The Netherlands.
Jason SuetersDelft University of Technology, Department of Imaging Physics, 2628 CD, Delft, The Netherlands.
Tsion E AbrahamErasmus Medical Centre Rotterdam, Department of Pathology, Optical Imaging Centre, 3000 CA, Rotterdam, The Netherlands.
Johan A SlotmanErasmus Medical Centre Rotterdam, Department of Pathology, Optical Imaging Centre, 3000 CA, Rotterdam, The Netherlands.
Gert-Jan KremersErasmus Medical Centre Rotterdam, Department of Pathology, Optical Imaging Centre, 3000 CA, Rotterdam, The Netherlands.
Jacob P HoogenboomDelft University of Technology, Department of Imaging Physics, 2628 CD, Delft, The Netherlands.
Adriaan B HoutsmullerErasmus Medical Centre Rotterdam, Department of Pathology, Optical Imaging Centre, 3000 CA, Rotterdam, The Netherlands.ORCID 0000-0003-0967-0740
Erasmus University Rotterdam · NLDelft University of Technology · NL

Funding

Erasmus Universiteit Rotterdam
6 · The paper itself

Abstract

Focal adhesions (FAs) are the main cellular structures to link the intracellular cytoskeleton to the extracellular matrix. FAs mediate cell adhesion, are important for cell migration and are involved in many (patho)-physiological processes. Here we examined FAs and their associated actin fibres using correlative fluorescence and scanning electron microscopy (SEM). We used fluorescence images of cells expressing paxillin-GFP to define the boundaries of FA complexes in SEM images, without using SEM contrast enhancing stains. We observed that SEM contrast was increased around the actin fibre entry site in 98% of FAs, indicating increases in protein density and possibly also phosphorylation levels in this area. In nearly three quarters of the FAs, these nanostructures had a fork shape, with the actin forming the stem and the high-contrast FA areas the fork. In conclusion, the combination of fluorescent and electron microscopy allowed accurate localisation of a highly abundant, novel fork structure at the FA-actin interface.

Indexed as

ActinsFocal AdhesionsCell AdhesionCytoskeletonMicroscopy, ElectronActinsCell migrationCorrelative microscopyElectron microscopyFluorescence microscopyFocal adhesions

Identifiers

PMID36409550
PMCPMC9836080
OpenAlexW4309667496

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.