ArticlemBio2022
Role of the Two Flagellar Stators in Swimming Motility of Pseudomonas putida.
Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Swimming patterns of a multi-mode bacterial swimmer in fluid shear flow.Biophysical journal · 2026Article
- Dual chemotaxis strategy of bacteria in porous media: Run-time and turn-angle bias.Biophysical journal · 2026Article
- Repurposing Tirazone as an effective quorum-sensing inhibitor against Pseudomonas aeruginosa virulence and biofilm formation.The Journal of antibiotics · 2026Article
- Article
- Bacterial swimming in porous gels exhibits intermittent run motility with active turns and mechanical trapping.Scientific reports · 2025Article
- Flagellar motility and the mucus environment influence aggregation-mediated antibiotic tolerance ofmBio · 2025Article
- PelD is required downstream of c-di-GMP for host specialization of Pseudomonas lurida.BMC microbiology · 2025Article
- Decoding Bacterial Motility: From Swimming States to Patterns and Chemotactic Strategies.Biomolecules · 2025Review
- Genome-wide identification of novel flagellar motility genes inFrontiers in microbiology · 2025Article
- The surface interface and swimming motility influence surface-sensing responses inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Manipulation of artificial and living small objects by light driven diffusioosmotic flow.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the soil bacterium Pseudomonas putida, the motor torque for flagellar rotation is generated by the two stators MotAB and MotCD. Here, we construct mutant strains in which one or both stators are knocked out and investigate their swimming motility in fluids of different viscosity and in heterogeneous structured environments (semisolid agar). Besides phase-contrast imaging of single-cell trajectories and spreading cultures, dual-color fluorescence microscopy allows us to quantify the role of the stators in enabling P. putida's three different swimming modes, where the flagellar bundle pushes, pulls, or wraps around the cell body. The MotAB stator is essential for swimming motility in liquids, while spreading in semisolid agar is not affected. Moreover, if the MotAB stator is knocked out, wrapped mode formation under low-viscosity conditions is strongly impaired and only partly restored for increased viscosity and in semisolid agar. In contrast, when the MotCD stator is missing, cells are indistinguishable from the wild type in fluid experiments but spread much more slowly in semisolid agar. Analysis of the microscopic trajectories reveals that the MotCD knockout strain forms sessile clusters, thereby reducing the number of motile cells, while the swimming speed is unaffected. Together, both stators ensure a robust wild type that swims efficiently under different environmental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.