ArticleThe Plant cell2023
Proton exchange by the vacuolar nitrate transporter CLCa is required for plant growth and nitrogen use efficiency.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Enhanced Activity of Glutamine Synthetase by Manipulating a GWAS-Identified BnaA02.GLN1;2 Gene Augments Nitrate Uptake and Yield in Brassica napus.Plant, cell & environment · 2026Article
- Research Progress on Chloride Channel- and Transporter- Related Gene Families in Plants.International journal of molecular sciences · 2026Review
- Identification, expression and potential functional analyses of the seven chloride channels in grapevine.Protoplasma · 2026Article
- Article
- PCLPred: identifying plant chloride transport-related proteins using reduced amino acid alphabets and N-peptide composition.Amino acids · 2026Article
- The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Homeostasis of ammonium and nitrate in plants.Quantitative plant biology · 2026Review
- Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants.Plant & cell physiology · 2025Article
- Rapeseed transcription factor BnaC9bHLH35 promotes root growth and improves nitrogen use efficiency under low nitrate conditions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- CLC gene family inFrontiers in plant science · 2025Article
- The crosstalk between nitrate signaling and other signaling molecules inFrontiers in plant science · 2025Review
- Chloride transport and homeostasis in plants.Quantitative plant biology · 2025Review
- Changes in the nitrate assimilation pathway serve as a component of maize stress response to competition cues.Frontiers in plant science · 2025Article
- Transcriptomic Analysis Reveals the Effect of Urea on Metabolism ofLife (Basel, Switzerland) · 2024Article
- Molecular mechanism underlying regulation of Arabidopsis CLCa transporter by nucleotides and phospholipids.Nature communications · 2023Article
- Chloride Channel Family in the EuhalophyteInternational journal of molecular sciences · 2023Article
- Focus on climate change and plant abiotic stress biology.The Plant cell · 2023Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrate is a major nutrient and osmoticum for plants. To deal with fluctuating nitrate availability in soils, plants store this nutrient in their vacuoles. Chloride channel a (CLCa), a 2NO3-/1H+ exchanger localized to the vacuole in Arabidopsis (Arabidopsis thaliana), ensures this storage process. CLCa belongs to the CLC family, which includes anion/proton exchangers and anion channels. A mutation in a glutamate residue conserved across CLC exchangers is likely responsible for the conversion of exchangers to channels. Here, we show that CLCa with a mutation in glutamate 203 (E203) behaves as an anion channel in its native membrane. We introduced the CLCaE203A point mutation to investigate its physiological importance into the Arabidopsis clca knockout mutant. These CLCaE203A mutants displayed a growth deficit linked to the disruption of water homeostasis. Additionally, CLCaE203A expression failed to complement the defect in nitrate accumulation of clca and favored higher N-assimilation at the vegetative stage. Further analyses at the post-flowering stages indicated that CLCaE203A expression results in an increase in N uptake allocation to seeds, leading to a higher nitrogen use efficiency compared to the wild-type. Altogether, these results point to the critical function of the CLCa exchanger on the vacuole for plant metabolism and development.
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