ArticleNucleic acids research2022
The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Bacterial 3D genome architecture: organization, regulation, and synthetic biology applications.Genome biology · 2026Review
- Reversible DNA condensation drives natural transformation.Nature communications · 2026Article
- An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales.Communications biology · 2025Article
- Gene loci fluctuations reveal bacterial chromosome dynamical organization.Communications biology · 2025Article
- Cyclic-di-GMP interferes with DNA-MucR-DNA bridging to derepress genes targeted by the xenogeneic silencer MucR.Nucleic acids research · 2025Article
- Genome-wide DNA bridging by H-NS reshapes the stationary phase nucleoid and transcriptional landscape.bioRxiv : the preprint server for biology · 2025Article
- Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC.Nucleic acids research · 2025Article
- Principles of bacterial genome organization, a conformational point of view.Molecular microbiology · 2025Review
- Identification, characterization and classification of prokaryotic nucleoid-associated proteins.Molecular microbiology · 2025Review
- Rok from B. subtilis: Bridging genome structure and transcription regulation.Molecular microbiology · 2025Review
- High-throughput single-molecule nanofluidic studies onQRB discovery · 2025Article
- Subtilosin A production is influenced by surfactin levels inmicroLife · 2025Article
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Lysine acetylation regulates the AT-rich DNA possession ability of H-NS.Nucleic acids research · 2024Article
- In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.Nature communications · 2023Article
- DNA-bridging by an archaeal histone variant via a unique tetramerisation interface.Communications biology · 2023Article
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11 authors.
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Abstract
Nucleoid-associated proteins (NAPs) play a central role in chromosome organization and environment-responsive transcription regulation. The Bacillus subtilis-encoded NAP Rok binds preferentially AT-rich regions of the genome, which often contain genes of foreign origin that are silenced by Rok binding. Additionally, Rok plays a role in chromosome architecture by binding in genomic clusters and promoting chromosomal loop formation. Based on this, Rok was proposed to be a functional homolog of E. coli H-NS. However, it is largely unclear how Rok binds DNA, how it represses transcription and whether Rok mediates environment-responsive gene regulation. Here, we investigated Rok's DNA binding properties and the effects of physico-chemical conditions thereon. We demonstrate that Rok is a DNA bridging protein similar to prototypical H-NS-like proteins. However, unlike these proteins, the DNA bridging ability of Rok is not affected by changes in physico-chemical conditions. The DNA binding properties of the Rok interaction partner sRok are affected by salt concentration. This suggests that in a minority of Bacillus strains Rok activity can be modulated by sRok, and thus respond indirectly to environmental stimuli. Despite several functional similarities, the absence of a direct response to physico-chemical changes establishes Rok as disparate member of the H-NS family.
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