ArticleFrontiers in microbiology2022
Assessment of shelf-life and metabolic viability of a multi-strain synbiotic using standard and innovative enumeration technologies.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Effect of probiotic lozenges on improvement in tympanometric classification within 12 weeks in children aged 3-6 years with adenoid hypertrophy and otitis media with effusion undergoing non-surgical management: a randomized controlled trial.Frontiers in cellular and infection microbiology · 2025Trial
- Revisiting Strategies for Bacterial Enumeration: The Case for Viable but Nonculturable (VBNC) Cells in Supplement Products.Probiotics and antimicrobial proteins · 2026Review
- Microbial Biofertilizers for Salinity Stress Mitigation in Hydroponic Systems.Current issues in molecular biology · 2025Review
- Redefining acne treatment: emerging role of probiotics in dermatology.Archives of microbiology · 2025Review
- Harnessing Probiotics to Combat Candidiasis: Mechanisms, Evidence, and Future Directions.Journal of fungi (Basel, Switzerland) · 2025Review
- Comparative Effects of Probiotics and Paraprobiotics Derived fromJournal of microbiology and biotechnology · 2025Article
- Proof of concept: real-time viability and metabolic profiling of probiotics with isothermal microcalorimetry.Frontiers in microbiology · 2024Article
- Specific cultivation-independent enumeration of viable cells in probiotic products using a combination of fluorescenceFrontiers in microbiology · 2024Article
- Dynamics of Active Fluorescent Units (AFU) and Water Activity (aFoods (Basel, Switzerland) · 2023Article
- Probiotic and postbiotic analytical methods: a perspective of available enumeration techniques.Frontiers in microbiology · 2023Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The number of live bacterial cells is the most used parameter to assess the quality of finished probiotic products. Plate counting (PC) is the standard method in industry to enumerate cells. Application of PC implies critical aspects related to the selection of optimal nutrient media and growth conditions and underestimation of viable but not cultivable (VBNC) cells. Flow-cytometry (FC) is a culture-independent methodology having the potential to selectively enumerate live, damaged, and dead cells representing a powerful tool for in-depth monitoring of probiotic products. We monitored the shelf life of a clinical batch of a synbiotic composition PDS-08 targeting the pediatric population by means of PC and FC according to International Conference on Harmonization (ICH) pharma guidelines testing the Arrhenius model as predictive tool; PC enumeration revealed higher destruction rate than FC suggesting a faster reduction in cultivability than membrane integrity and thus a possible shift of the bacteria into a VBNC status. PDS-08 maintained acidification capability over time, when re-suspended in nutrient medium, even in samples tested sub-optimally for CFU detection (below 1 billion cells/dose). Due to similar kinetics described by the study of metabolic activity and membrane integrity, FC might be suggested as a valid tool for the study of functional stability of a probiotic product.
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