ArticlePlant biotechnology journal2023
CRISPR-induced miRNA156-recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 75 citations in OpenAlex.
- Harnessing microRNA regulatory networks for engineering climate resilience and nutritional enhancement in millets.Stress biology · 2026Review
- Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding.The New phytologist · 2026Review
- Wheat TaSPL13-2B Improves Floret Fertility and Enhances Grain Number per Spikelet Through Jasmonic Acid Signalling Pathway.Plant biotechnology journal · 2026Article
- MicroRNA156 and its targeted SPL genes interact with the photoperiod, vernalization, and gibberellin pathways to regulate wheat heading time.The Plant journal : for cell and molecular biology · 2026Article
- Uncovering yield-related traits loci in Chinese wheat landraces using GWAS.Frontiers in plant science · 2026Article
- Integrated Multi-Omics Reveals the Molecular Basis Underlying Wheat Grain Development and IdentifiesPlants (Basel, Switzerland) · 2025Article
- Small RNAs big impact: a review on microRNA-mediated tolerance in wheat under terminal heat.Functional & integrative genomics · 2025Review
- From convention to innovation: the role of genetic modification and genome editing in Australian wheat breeding.AoB PLANTS · 2025Review
- Gluten Proteins: Beneficial Factors and Toxic Triggers in Human Health.Foods (Basel, Switzerland) · 2025Review
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Recent advances in improving yield and immunity through transcription factor engineering.Journal of integrative plant biology · 2025Review
- The selection and application of tiller number QTLs in modern wheat breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Transcriptome and Weighted Gene Co-Expression Network Analysis to Characterize the Expression of Genes Related to Yield Traits in Yunnan Hulled Wheat.International journal of molecular sciences · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Genetic and molecular insights into tiller development and approaches for crop yield improvement.Frontiers in plant science · 2025Review
- Integrative Transcriptomic and Small RNA Analysis Uncovers Key Genes for Cold Resistance in Rice.Genes · 2024Article
- Genome-Wide Identification and Expression Profiling of thePlants (Basel, Switzerland) · 2024Article
- Characterization of tae-miR156(s) and their response to abiotic stress in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- Identification and Functional Studies on the Role ofInternational journal of molecular sciences · 2024Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increase in grain yield is always a major objective of wheat genetic improvement. The SQUAMOSA promoter-binding protein-like (SPL) genes, coding for a small family of diverse plant-specific transcription factors, represent important targets for improving grain yield and other major agronomic traits in rice. The function of the SPL genes in wheat remains to be investigated in this respect. In this study, we identified 56 wheat orthologues of rice SPL genes belonging to 19 homoeologous groups. Like in rice, nine orthologous TaSPL genes harbour the microRNA156 recognition elements (MRE) in their last exons except for TaSPL13, which harbour the MRE in its 3'-untranslated region (3'UTR). We modified the MRE of TaSPL13 using CRISPR-Cas9 and generated 12 mutations in the three homoeologous genes. As expected, the MRE mutations led to an approximately two-fold increase in the TaSPL13 mutant transcripts. The phenotypic evaluation showed that the MRE mutations in TaSPL13 resulted in a decrease in flowering time, tiller number, and plant height, and a concomitantly increase in grain size and number. The results show that the TaSPL13 mutants exhibit a combination of different phenotypes observed in Arabidopsis AtSPL3/4/5 mutants and rice OsSPL13/14/16 mutants and hold great potential in improving wheat yield by simultaneously increasing grain size and number and by refining plant architecture. The novel TaSPL13 mutations generated can be utilized in wheat breeding programmes to improve these agronomic traits.
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