ArticleThe Plant journal : for cell and molecular biology2023
A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19.
Article in The Plant journal : for cell and molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed.
- Virus-Induced Gene Silencing for Nodulation Analysis in Soybean.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- A receptor kinase complex refines cambium activity inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- A synthetic expression system for orthogonal gene expression in Nicotiana benthamiana.Plant molecular biology · 2026Article
- Dual recognition of structurally unrelated mildew effectors underlies the broad-spectrum resistance of Pm3e in wheat.Nature communications · 2026Article
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- Virus-induced genome editing enables functional genomics across diverse plant species.Molecules and cells · 2026Review
- Cell-type-specific ablation of siRNAs by Arabidopsis RTL1 reveals a role of phloem companion cells in systemic post-transcriptional gene silencing.Nucleic acids research · 2026Article
- Strain, procedures, and tools for reproducible genetic transformation and genome editing of the emerging plant model Spirodela polyrhiza.The New phytologist · 2026Article
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
- Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Seamless, modular binary vectors for plant cell and molecular biology.Plant biotechnology journal · 2026Article
- Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors.Nature plants · 2026Article
- Plant-Based Ligand-Depletion Binding Assay for Assessing Pattern Recognition Receptor-Ligand Interactions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Functional diversity of sunflower DGAT1 and DGAT2 enzymes underlying seed triacylglycerol biosynthesis.Frontiers in plant science · 2026Article
- A SUMO interacting motif in the replication initiator protein of tomato yellow leaf curl virus is required for viral replication.Journal of virology · 2025Article
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- The arabidopsis GyraseB3 contributes to transposon silencing by promoting histone deacetylation.Nucleic acids research · 2025Article
- Tomato Bushy Stunt Virus (TBSV): From a Plant Pathogen to a Multifunctional Biotechnology Platform.Viruses · 2025Review
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3 authors.
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Abstract
Transient transgenic expression accelerates pharming and facilitates protein studies in plants. One embodiment of the approach involves leaf infiltration of Agrobacterium strains whose T-DNA is engineered with the gene(s) of interest. However, gene expression during 'agro-infiltration' is intrinsically and universally impeded by the onset of post-transcriptional gene silencing (PTGS). Nearly 20 years ago, a simple method was developed, whereby co-expression of the tombusvirus-encoded P19 protein suppresses PTGS and thus enhances transient gene expression. Yet, how PTGS is activated and suppressed by P19 during the process has remained unclear to date. Here, we address these intertwined questions in a manner also rationalizing how vastly increased protein yields are achieved using a minimal viral replicon as a transient gene expression vector. We also explore, in side-by-side analyses, why some proteins do not accumulate to the expected high levels in the assay, despite vastly increased mRNA levels. We validate that enhanced co-expression of multiple constructs is achieved within the same transformed cells, and illustrate how the P19 system allows rapid protein purification for optimized downstream in vitro applications. Finally, we assess the suitability of the P19 system for subcellular localization studies - an originally unanticipated, yet increasingly popular application - and uncover shortcomings of this specific implement. In revisiting the P19 system using contemporary knowledge, this study sheds light onto its hitherto poorly understood mechanisms while further illustrating its versatility but also some of its limits.
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