Evidence map›Paper›PMID 36401176›Full record

ArticleMolecular medicine (Cambridge, Mass.)2022

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway.

Ansong Liu, Yong Chen, Da Zhong, Chenggong Wang, Mi Yu, Chao Liu, Zhijun Yang, Wenkang Chen, Ke Yin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Circular RNAs: Promising Targets in Osteoporosis.Current osteoporosis reports · 2023
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Ansong Liu *The First Affiliated Hospital, Department of Orthopedics, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China.
Yong Chen *The First Affiliated Hospital, Department of Spine, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China.
Da ZhongDepartment of Orthopedics, Xiangya Hospital, Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China.
Chenggong WangDepartment of Orthopedics, Xiangya Hospital, Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China.
Mi YuHengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Chao LiuThe First Affiliated Hospital, Department of Orthopedics, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China.
Zhijun YangThe First Affiliated Hospital, Department of Orthopedics, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China.
Wenkang ChenThe First Affiliated Hospital, Department of Orthopedics, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China.
Ke YinThe First Affiliated Hospital, Department of Orthopedics, Hengyang Medical School, University of South China, No. 69 Chuanshan Road, Hengyang, Hunan, 421001, China. ink2000_2000@sina.com.ORCID 0000-0002-8196-1377
University of South China · CNCentral South University · CNFirst Affiliated Hospital of University of South China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNA (circ) AFF4 was documented to regulate osteogenesis but the underlying mechanism remains to be elucidated. The preliminary study showed that circ_AFF4 may promote osteogenesis via FNDC5/Irisin. Furthermore, the online prediction tool indicated the interaction of circ_AFF4, insulin-like growth factor-2 mRNA-binding protein 3 (IGF2BP3), FNDC5 and lysine (K)-specific demethylase 1 A (KDM1A). Therefore, this study aims to elucidate the relationships of KDM1A, circ_AFF4, IGF2BP3 and FNDC5/Irisin during osteogenesis.

methodsThe alkaline phosphatase (ALP) activities and osteogenic-related factors were determined using ALP and alizarin red S (ARS) staining, real-time quantitative PCR(RT-qPCR) and western blot. Immunoprecipitation (RIP), pull-down assay and fluorescence in situ hybridization (FISH) were used to examine the interactions among circ_AFF4/IGF2BP3/FNDC5. A mouse in vivo model was utilized to further confirm the regulatory effect on bone formation.

resultsCirc_AFF4 and KDM1A expression levels were increased during osteoinduction of BM-MSCs. Knockdown of circ_AFF4 and KDM1A significantly suppressed BM-MSC osteogenesis. We also proved that KDM1A directly bound to circ_AFF4 and FNDC5 promoter and induced circ_AFF4 and FNDC5 expression. Furthermore, circ_AFF4 enhanced the stability of FNDC5 by generating a circ_AFF4, IGF2BP3 and FNDC5 RNA-protein complex, and thereby induced Irisin and osteogenesis. The in vitro data was confirmed with in vivo model.

conclusionThese findings elucidate that KDM1A induces circ_AFF4, which promotes promote osteogenesis via IGF2BP3. This study indicates that circ_AFF4 may potentially represent a critical therapeutic target for the diseases.

Indexed as

OsteogenesisRNA, CircularAnimalsFibronectinsIn Situ Hybridization, FluorescenceMiceTranscription FactorsFibronectinsFNDC5 protein, mouseRNA, CircularTranscription FactorsCircular RNA AFF4FNDC5IrisinKDM1AOsteogenic differentiation

Identifiers

PMID36401176
PMCPMC9673395
OpenAlexW4309288208

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.