Evidence map›Paper›PMID 36401045›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

High-Throughput Screening to Identify Modulators of Sarcospan.

Cynthia Shu, Ekaterina Mokhonova, Rachelle H Crosbie

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Cynthia ShuDepartment of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, USA.
Ekaterina MokhonovaDepartment of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, USA.
Rachelle H CrosbieDepartment of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, USA. rcrosbie@physci.ucla.edu.
University of California, Los Angeles · US

Funding

Structure-Function Analysis of SarcospanR01AR048179 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2001 to 2023
$6.9M
UCLA Muscular Dystrophy Core CenterP30AR057230 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SPENCER, MELISSA JAN · 2009 to 2018
$6.2M
Muscle Cell Biology, Pathophysiology, and TherapeuticsT32AR065972 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Rachelle Hope Crosbie, THOMAS A. RANDO · 2016 to 2026
$3.9M
Restoration of muscle cell adhesion to treat cardiomyopathy in muscular dystrophyR01HL126204 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2016 to 2019
$1.5M
NHLBI NIH HHS R01 HL126204NIAMS NIH HHS P30 AR057230NIAMS NIH HHS R01 AR048179NIAMS NIH HHS T32 AR065972
6 · The paper itself

Abstract

High-throughput screening enables the discovery of disease-modifying small molecules. Here, we describe the development of a scalable, cell-based assay to screen for small molecules that modulate sarcospan for the treatment of Duchenne muscular dystrophy. We detail the hit validation pipeline, which includes secondary screening, gene/protein quantification, and an in vitro membrane stability assay.

Indexed as

High-Throughput Screening AssaysMuscular Dystrophy, DuchenneHumansDrug discoveryDuchenne muscular dystrophyHigh-throughput screeningSarcospan

Identifiers

PMID36401045
OpenAlexW4309329621

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.