Evidence map›Paper›PMID 36401028›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Restoring Dystrophin Expression with Duchenne Muscular Dystrophy Exon 45 Skipping in Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Mitsuto Sato, Naoko Shiba, Daigo Miyazaki, Yuji Shiba, Akinori Nakamura

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 67% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Mitsuto SatoDepartment of Medicine (Neurology and Rheumatology) , Shinshu University School of Medicine, Matsumoto, Japan.
Naoko ShibaDepartment of Regenerative Science and Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Daigo MiyazakiDepartment of Medicine (Neurology and Rheumatology) , Shinshu University School of Medicine, Matsumoto, Japan.
Yuji ShibaDepartment of Regenerative Science and Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Akinori NakamuraDepartment of Medicine (Neurology and Rheumatology) , Shinshu University School of Medicine, Matsumoto, Japan. anakamu@shinshu-u.ac.jp.
Shinshu University · JPNational Hospital Organization · JPShinshu University Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cell (iPSC)-based disease model is a useful tool that can represent the pathophysiology of patient organs that are inaccessible due to invasiveness. Here, we present a method to induce differentiation of Duchenne muscular dystrophy (DMD) patient-derived iPSCs into cardiomyocytes and restore dystrophin expression by exon skipping using antisense nucleic acids. This involves a 20-day multi-step culture process for differentiation to cardiomyocytes, followed by exon-skipping experiments. Additionally, RT-PCR, western blotting, and immunocytochemistry are used to confirm the restoration of dystrophin expression.

Indexed as

Induced Pluripotent Stem CellsMuscular Dystrophy, DuchenneDystrophinExonsHumansMyocytes, CardiacDystrophinAntisense oligonucleotide (ASO)CardiomyocytesCell cultureDuchenne muscular dystrophy (DMD)Exon skipImmunocytochemistryInduced pluripotent stem cells (iPSCs)RT-PCRWestern blotting

Identifiers

PMID36401028
OpenAlexW4309329496

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.