ArticleNature communications2022
Donor-strand exchange drives assembly of the TasA scaffold in Bacillus subtilis biofilms.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
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- Disrupting phage liquid crystalline droplets restores antibiotic susceptibility in Pseudomonas aeruginosa biofilms.PLoS biology · 2026Article
- Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly.Nature communications · 2026Article
- Metabolomics aided by machine learning decodes adaptive remodeling of Bacillus biofilms in response to pasteurization stress.NPJ science of food · 2026Article
- Precision Labeling of Native Antibodies with Lock Coupling.Journal of the American Chemical Society · 2026Article
- The Many Lives of a Single Sequence: Functional Plasticity Through Amyloid Polymorphism.Sub-cellular biochemistry · 2026Review
- The Importance of Being Imperfect: Structure and Function of Bacterial Amyloid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- eDNA-Amyloid Synergistic Interactions in Bacterial Biofilms: A Hidden Driver of Antimicrobial Resistance.International journal of molecular sciences · 2025Review
- Dental plaque biofilm transforms host-derived βBiofilm · 2025Article
- Effective disposal and remediation of chemical agents with designer living biofilm materials in soil and water.Fundamental research · 2025Article
- Donor strand complementation and calcium ion coordination drive the chaperone-free polymerization of archaeal cannulae.Nature communications · 2025Article
- Structural basis ofScience advances · 2025Article
- Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes.Nature communications · 2025Article
- Tat-dependent bundling pilus of a halophilic archaeon assembles by a strand donation mechanism and facilitates biofilm formation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cloning, Heterologous Expression, and Antifungal Activity Evaluation of a Novel Truncated TasA Protein fromInternational journal of molecular sciences · 2025Article
- Targeted disruption of phage liquid crystalline droplets abolishes antibiotic tolerance of bacterial biofilms.bioRxiv : the preprint server for biology · 2025Article
- Structure of the virulence-associatedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular choreography of sludge extracellular polymeric substances-From biomolecule identification to energetics and assembly dynamics.PNAS nexus · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Many bacteria in nature exist in multicellular communities termed biofilms, where cells are embedded in an extracellular matrix that provides rigidity to the biofilm and protects cells from chemical and mechanical stresses. In the Gram-positive model bacterium Bacillus subtilis, TasA is the major protein component of the biofilm matrix, where it has been reported to form functional amyloid fibres contributing to biofilm structure and stability. Here, we present electron cryomicroscopy structures of TasA fibres, which show that, rather than forming amyloid fibrils, TasA monomers assemble into fibres through donor-strand exchange, with each subunit donating a β-strand to complete the fold of the next subunit along the fibre. Combining electron cryotomography, atomic force microscopy, and mutational studies, we show how TasA fibres congregate in three dimensions to form abundant fibre bundles that are essential for B. subtilis biofilm formation. Our study explains the previously observed biochemical properties of TasA and shows how a bacterial extracellular globular protein can assemble from monomers into β-sheet-rich fibres, and how such fibres assemble into bundles in biofilms.
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