ArticleACS sensors2022
CRISPR/Cas-Assisted Colorimetric Biosensor for Point-of-Use Testing for African Swine Fever Virus.
Article in ACS sensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 51 citations in OpenAlex.
- Recent Advances in CRISPR/Cas Technologies for Biological Discovery, Therapeutics, and Diagnostics.Biomolecules · 2026Review
- Biosensors for the detection of arthropod-borne veterinary viruses: A comprehensive review.Veterinary and animal science · 2026Review
- Programmable CRISPR-mediated gold nanoparticle adhesion for visual colorimetric detection.Biosensors & bioelectronics · 2026Article
- Hybridization chain reaction-assisted CRISPR/Cas12a strategy for rapid and visual detection ofFrontiers in cellular and infection microbiology · 2026Article
- A multifunctional approach: merging CRISPR/Cas technology with DNA nanomachines for advanced biosensing.Analytical and bioanalytical chemistry · 2026Review
- Review
- ASF outbreaks in Vietnam (2019-2024): insights and lessons learned.Veterinary research communications · 2025Review
- Colorimetric Biosensors: Advancements in Nanomaterials and Cutting-Edge Detection Strategies.Biosensors · 2025Review
- A tube-based biosensor for DNA and RNA detection.Science advances · 2025Article
- Spatial prediction of wild boar distribution in Thailand applications for African swine fever prevention and control.Scientific reports · 2025Article
- Colorimetric CRISPR Biosensor: A Case Study with Salmonella Typhi.ACS sensors · 2025Article
- Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay for Point-of-Care Testing of Urinary Biomarkers.ACS sensors · 2024Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever virus (ASFV) causes a highly contagious and fatal disease affecting both domesticated and wild pigs. Substandard therapies and inadequate vaccinations cause severe economic damages from pig culling and removal of infected carcasses. Therefore, there is an urgent need to develop a rapid point-of-use approach that assists in avoiding the spread of ASFV and reducing economic loss. In this study, we developed a colorimetric sensing platform based on dual enzymatic amplification that combined the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a) system and the enzyme urease for accurate and sensitive detection of ASFV. The mechanism of the sensing platform involves a magnetic bead-anchored urease-conjugated single-stranded oligodeoxynucleotide (MB@urODN), which in the presence of ASFV dsDNA is cleaved by activated CRISPR/Cas12a. After magnetically separating the free urease, the presence of virus can be confirmed by measuring the colorimetric change in the solution. The advantage of this method is that it can detect the presence of virus without undergoing a complex target gene duplication process. The established method detected ASFV from three clinical specimens collected from porcine clinical tissue samples. The proposed platform is designed to provide an adequate, simple, robust, highly sensitive and selective analytical technique for rapid zoonotic disease diagnosis while eliminating the need for vast or specialized tools.
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