ArticleDevelopment (Cambridge, England)2022
Single-cell RNA-sequencing analysis of early sea star development.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Distinct cell states define larval and adult body plans in a hemichordate.Nature communications · 2026Article
- Marine Model Organisms for Mechanobiology Studies.Results and problems in cell differentiation · 2026Review
- Article
- Single-Cell Transcriptomics Reveals Evolutionary Reconfiguration of Embryonic Cell Fate Specification in the Sea Urchin Heliocidaris erythrogramma.Genome biology and evolution · 2025Article
- Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.bioRxiv : the preprint server for biology · 2024Article
- Single-cell transcriptomics reveals evolutionary reconfiguration of embryonic cell fate specification in the sea urchinbioRxiv : the preprint server for biology · 2024Article
- Marine Invertebrates One Cell at A Time: Insights from Single-Cell Analysis.Integrative and comparative biology · 2023Review
- Article
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Authors and funding
5 authors.
Funding
Abstract
Echinoderms represent a broad phylum with many tractable features to test evolutionary changes and constraints. Here, we present a single-cell RNA-sequencing analysis of early development in the sea star Patiria miniata, to complement the recent analysis of two sea urchin species. We identified 20 cell states across six developmental stages from 8 hpf to mid-gastrula stage, using the analysis of 25,703 cells. The clusters were assigned cell states based on known marker gene expression and by in situ RNA hybridization. We found that early (morula, 8-14 hpf) and late (blastula-to-mid-gastrula) cell states are transcriptionally distinct. Cells surrounding the blastopore undergo rapid cell state changes that include endomesoderm diversification. Of particular import to understanding germ cell specification is that we never see Nodal pathway members within Nanos/Vasa-positive cells in the region known to give rise to the primordial germ cells (PGCs). The results from this work contrast the results of PGC specification in the sea urchin, and the dataset presented here enables deeper comparative studies in tractable developmental models for testing a variety of developmental mechanisms.
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