ArticleNature methods2022
RS-FISH: precise, interactive, fast, and scalable FISH spot detection.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Core circadian clock genes control molecular and behavioral circatidal rhythms in Parhyale hawaiensis.Current biology : CB · 2026Article
- Human Y chromosome pan-organ mapping reveals progressive mosaic loss from normal to cancer.JCI insight · 2026Article
- SpotMAX: A generalist framework for multidimensional automatic spot detection and quantification.Science advances · 2026Article
- Imaging-Based Spatial Transcriptomics: Data Interpretation Methods and Biomedical Applications.Biology · 2026Review
- Locally synthesized glycyl aminoacyl-tRNA synthetase is important for local translation in neurons.Life science alliance · 2026Article
- Directed Evolution Improves the Catalytic Efficiency of APEX2-Mediated Proximity-Dependent RNA Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- zFISHer: Automated 3D Registration, Detection, and Colocalization with Interactive Curation for Sequential Multiplexed FISH.bioRxiv : the preprint server for biology · 2026Article
- Transcriptional noise sets fundamental limits to decoding circadian clock phase from single-cell RNA snapshots.iScience · 2026Article
- autoFISH: a modular toolbox for sequential single-molecule RNA FISH experiments.Communications biology · 2026Article
- Multi-parametric longitudinal imaging of cerebral biomarkers in a rodent model of Alzheimer's disease.Photoacoustics · 2026Article
- A surface morphology-based inference method for the cell wall elasticity profile in tip-growing cells.PLoS computational biology · 2026Article
- Developing a Robust Multiround HCR-FISH Method Modified for Caenorhabditis elegans.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Applications and development of in situ nucleic acid visualization techniques.Frontiers in bioengineering and biotechnology · 2026Review
- Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotency.Nucleic acids research · 2025Article
- Spatiotemporal regulation of endoplasmic reticulum stress visualized by live imaging of bipA mRNA in Aspergillus oryzae.Scientific reports · 2025Article
- TrueSpot: a robust automated tool for quantifying signal puncta in fluorescent imaging.Genome biology · 2025Article
- Establishing a continuum of cell types in the visual cortex.bioRxiv : the preprint server for biology · 2025Article
- U-FISH: a fluorescent spot detector for imaging-based spatial-omics analysis and AI-assisted FISH diagnosis.Genome biology · 2025Article
- Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression.Nature methods · 2025Article
- Expression of clock genes tracks daily and tidal time in brains of intertidal crustaceans Eurydice pulchra and Parhyale hawaiensis.Current biology : CB · 2025Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Fluorescent in-situ hybridization (FISH)-based methods extract spatially resolved genetic and epigenetic information from biological samples by detecting fluorescent spots in microscopy images, an often challenging task. We present Radial Symmetry-FISH (RS-FISH), an accurate, fast, and user-friendly software for spot detection in two- and three-dimensional images. RS-FISH offers interactive parameter tuning and readily scales to large datasets and image volumes of cleared or expanded samples using distributed processing on workstations, clusters, or the cloud. RS-FISH maintains high detection accuracy and low localization error across a wide range of signal-to-noise ratios, a key feature for single-molecule FISH, spatial transcriptomics, or spatial genomics applications.
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