Observational studyFrontiers in cellular and infection microbiology2022
Diagnostic value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in pediatric pneumonia.
Observational study in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 22 citations in OpenAlex.
- Clinical impact of metagenomic next-generation sequencing for pathogen identification and guided therapy in pediatric intensive care unit patients with severe pulmonary infections.Microbiology spectrum · 2026Article
- Risk factors for plastic bronchitis in children with community-acquired pneumonia: a retrospective bronchoscopy-based study.BMC pulmonary medicine · 2026Article
- Comparative study of targeted next-generation sequencing and traditional pathogen detection methods in lower respiratory tract infections: impact on patient outcomes.Frontiers in microbiology · 2026Article
- Next-generation sequencing in the etiological diagnosis of severe childhood pneumonia.Frontiers in pediatrics · 2026Review
- Case report: novel NFKB2 variant associated with pediatric eosinophilic granulomatosis with polyangiitis (EGPA) in the COVID-19 pandemic.Pediatric rheumatology online journal · 2025Article
- Clinical diagnostic value of throat swabs in pediatric acute lower respiratory tract infections using targeted next-generation sequencing.BMC pediatrics · 2025Article
- Diagnostic value of metagenomic next-generation sequencing using bronchoalveolar lavage fluid samples for pathogen detection in children with severe or refractory pneumonia.Microbiology spectrum · 2025Article
- Explore the Application Value of Metagenomic Next-Generation Sequencing of Bronchoalveolar Lavage Fluid in the Early Diagnosis of Pulmonary Tuberculosis.Infection and drug resistance · 2025Article
- The impact of bronchoalveolar lavage fluid metagenomics next-generation sequencing on the diagnosis and management of patients with suspected pulmonary infection.Frontiers in cellular and infection microbiology · 2025Article
- Probe-based metagenomic pathogen detection: advancing laboratory capacity for complex diagnosis.Frontiers in microbiology · 2025Article
- Diagnostic utility of metagenomic next-generation sequencing for tissue in patients with suspected infectious diseases.Frontiers in cellular and infection microbiology · 2025Article
- Risk factors and prescription patterns analysis for macrolide-resistant Mycoplasma pneumoniae pneumonia in children.iScience · 2024Article
- Comprehensive human respiratory genome catalogue underlies the high resolution and precision of the respiratory microbiome.Briefings in bioinformatics · 2024Article
- Microbiological Profile of Patients with Aspiration Pneumonia Identified by Combined Detection Methods.Infection and drug resistance · 2024Article
- Application of metagenomic next-generation sequencing in the etiological diagnosis of refractory pneumonia in children.Frontiers in microbiology · 2024Article
- Metagenomic next-generation sequencing targeted and metagenomic next-generation sequencing for pulmonary infection in HIV-infected and non-HIV-infected individuals.Frontiers in cellular and infection microbiology · 2024Article
- Utilizing metagenomic next-generation sequencing for diagnosis and lung microbiome probing of pediatric pneumonia through bronchoalveolar lavage fluid in pediatric intensive care unit: results from a large real-world cohort.Frontiers in cellular and infection microbiology · 2023Article
- Validation of metagenomic next-generation sequencing of bronchoalveolar lavage fluid for diagnosis of suspected pulmonary infections in patients with systemic autoimmune rheumatic diseases receiving immunosuppressant therapy.Frontiers in medicine · 2023Article
- Diagnostic performance and clinical impact of blood metagenomic next-generation sequencing in ICU patients suspected monomicrobial and polymicrobial bloodstream infections.Frontiers in cellular and infection microbiology · 2023Article
- Clinical metagenomics-challenges and future prospects.Frontiers in microbiology · 2023Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objectives: The aim of this study was to evaluate the diagnostic value of bronchoalveolar lavage fluid (BALF) metagenomic next-generation sequencing (mNGS) versus conventional microbiological tests (CMTs) for pediatric pneumonia. Methods: This retrospective observational study enrolled 103 children who were diagnosed with pneumonia and hospitalized at Hubei Maternity and Child Health Care Hospital between 15 October 2020 and 15 February 2022. The pneumonia diagnosis was based on clinical manifestations, lung imaging, and microbiological tests. Pathogens in the lower respiratory tract were detected using CMTs and BALF mNGS (of DNA and RNA). The diagnostic performance of BALF mNGS was compared with that of CMTs. Results: In 96 patients, pathogens were identified by microbiological tests. The overall pathogen detection rate of mNGS was significantly higher than that of CMTs (91.3% vs. 59.2%, Conclusions: BALF mNGS improved the detection rate of pediatric pneumonia, especially in mixed infections. The diagnostic performance of BALF mNGS varies according to pathogen type. mNGS can be used to supplement CMTs. A combination of mNGS and CMTs may be the best diagnostic strategy.
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