Evidence map›Paper›PMID 36386881›Full record

ArticleSTAR protocols2022

Sparse postnatal labeling and quantification of superficial cortical cell synapses in the mouse neocortex.

Lorenzo Gesuita, Ali Özgür Argunsah, Theofanis Karayannis

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lorenzo GesuitaLaboratory of Neural Circuit Assembly, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich (ZNZ), 8057 Zurich, Switzerland.
Ali Özgür ArgunsahLaboratory of Neural Circuit Assembly, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich (ZNZ), 8057 Zurich, Switzerland. Electronic address: argunsah@hifo.uzh.ch.
Theofanis KarayannisLaboratory of Neural Circuit Assembly, Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland; Neuroscience Center Zurich (ZNZ), 8057 Zurich, Switzerland. Electronic address: karayannis@hifo.uzh.ch.
University of Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a strategy for measuring the density of presynaptic boutons of superficial neuronal cells in the mouse neocortex. First, we show how to sparsely label individual postmitotic cells by neonatal pial-surface electroporation. Then, we present a custom-made code that allows quantification of the density of presynaptic boutons along axonal processes. Although we applied this strategy to somatostatin-positive cells, a major population of cortical interneurons, the same approach can be adjusted to target other types of neuronal cells. For complete details on the use and execution of this protocol, please refer to Gesuita et al. (2022).

Indexed as

NeocortexAnimalsAxonsMiceNeuronsPresynaptic TerminalsSynapsesBioinformaticsDevelopmental biologyMicroscopyMolecular BiologyNeuroscience

Identifiers

PMID36386881
PMCPMC9663983
OpenAlexW4308787948

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.