Evidence map›Paper›PMID 36386869›Full record

ArticleSTAR protocols2022

Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation.

Gavin I Ellis, Mosha Z Deng, Delaine W Winn, Kimberly E Coker, Divanshu Shukla, Vijay Bhoj, Michael C Milone, Raimon Duran-Struuck, James L Riley

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Tolerogenic Therapies in Cardiac Transplantation.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Gavin I EllisDepartment of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA.
Mosha Z DengDepartment of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA.
Delaine W WinnDepartment of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA.
Kimberly E CokerDepartment of Pathobiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Divanshu ShuklaDepartment of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA.
Vijay BhojDeparment of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Michael C MiloneDeparment of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Raimon Duran-StruuckDepartment of Pathobiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
James L RileyDepartment of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: rileyj@upenn.edu.
University of Pennsylvania · US

Funding

Microphysiological systems for modeling autoimmunity in type 1 diabetesUH3DK122644 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI GADUE, PAUL J, HUH, DONGEUN · 2021 to 2023
$3.4M
Engineering T cells to Promote Islet TransplantR01DK132725 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI NAJI, ALI, RILEY, JAMES L. · 2022 to 2025
$3.1M
NIDDK NIH HHS R01 DK132725NIDDK NIH HHS UH3 DK122644
6 · The paper itself

Abstract

It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-presenting cells, transduction by simian tropic lentiviral vectors, and antigen-specific expansion. The result of this method is highly suppressive CAR Tregs for use in a pre-clinical, large animal model of transplant tolerance. For complete details on the use and execution of this protocol, please refer to Ellis et al. (2022).

Indexed as

Receptors, Chimeric AntigenAnimalsAntigen-Presenting CellsPrimatesT-Lymphocytes, RegulatoryTransplantation ToleranceReceptors, Chimeric AntigenBiotechnology and bioengineeringCell cultureCell isolationFlow cytometry/Mass cytometryImmunologyModel organismsTissue engineering

Identifiers

PMID36386869
PMCPMC9641266
OpenAlexW4308266083

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.