ArticleACS omega2022
Efficient and Simple Paper-Based Assay for Plasma Separation Using Universal Anti-H Agglutinating Antibody.
Article in ACS omega, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Emerging Microfluidic Plasma Separation Technologies for Point-of-Care Diagnostics: Moving Beyond Conventional Centrifugation.Biosensors · 2025Review
- Paper-Based Device for the Colorimetric Determination of Glucose in Whole-Blood Samples Using a Smartphone.Bioengineering (Basel, Switzerland) · 2025Article
- Plasma separation from whole blood on paper-based fluidic devices.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Capillary-driven distance-based paper analytical devices for albumin protein and glucose quantification in human whole blood.Mikrochimica acta · 2025Article
- Quantitative and equipment-free paper-based agglutination assay of bacterial cells.RSC advances · 2024Article
- One-Step Detection of Vancomycin in Whole Blood Using the Lateral Flow Immunoassay.Biosensors · 2024Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConventional laboratory tests require plasma separation using centrifugation by skilled personnel in well-equipped lab. Development of a simple, reliable, and cheap point-of-care (POC) test for plasma separation will overcome these limitations.
methodsPlasma separation was achieved in filter paper using the anti-H agglutinating antibody. Hydrophobic channels were created using a solid ink printer. The reproducibility, efficiency, recovery, and applicability of the assay were validated on a large number of blood samples.
resultsA simple, fast, cheap, and direct paper-based assay for plasma separation from whole blood using universal anti-H agglutinating antibody was developed without equipment or pretreatment requirements. The purity of plasma separation using anti-H treated paper was confirmed by microscopy and biuret test for plasma albumin detection. Plasma separation was affected by paper structure, antibody concentration, donor gender, and hematocrit. The efficiency of the assay was 72% and the reproducibility was about 90% with minimal interassay and intra-assay variabilities. The assay successfully separated plasma from 116/119 samples, indicating high sensitivity (97.5%). Furthermore, the assay accurately recovers thyroid stimulating hormone from samples compared to standard methods with 107% recovery rate.
conclusionsPaper-based plasma separation using anti-H agglutinating antibodies would have numerous applications in paper-based POC tests and in resource limited areas.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.