ArticleNature communications2022
Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq.Cell reports methods · 2026Article
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Protocol for mapping neural circuit connectivity with START: Single transcriptome assisted rabies tracing.STAR protocols · 2026Article
- Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice.STAR protocols · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- Connectome-seq: high-throughput mapping of neuronal connectivity at single-synapse resolution via barcode sequencing.Nature methods · 2026Article
- Neuro-Immune Crosstalk: Molecular Mechanisms, Biological Functions, Diseases, and Therapeutic Targets.MedComm · 2026Review
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons.Cell reports methods · 2025Article
- One-step approach producing barcoded rabies virus with optimized diversity.Cell reports methods · 2025Article
- Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Article
- Connectome-seq: High-throughput Mapping of Neuronal Connectivity at Single-Synapse Resolution via Barcode Sequencing.bioRxiv : the preprint server for biology · 2025Article
- Article
- Single-cell genomics meets systems neuroscience: Insights from mapping the brain circuitry of stress.Journal of neuroendocrinology · 2025Review
- Limitations and optimizations of cellular lineages tracking.PLoS computational biology · 2025Article
- The neuroimmune connectome in health and disease.Nature · 2025Review
- High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping Using Single-Cell and Single-Nucleus Sequencing.bioRxiv : the preprint server for biology · 2024Article
- Review
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Authors and funding
12 authors.
Funding
Abstract
Brain function depends on synaptic connections between specific neuron types, yet systematic descriptions of synaptic networks and their molecular properties are not readily available. Here, we introduce SBARRO (Synaptic Barcode Analysis by Retrograde Rabies ReadOut), a method that uses single-cell RNA sequencing to reveal directional, monosynaptic relationships based on the paths of a barcoded rabies virus from its "starter" postsynaptic cell to that cell's presynaptic partners. Thousands of these partner relationships can be ascertained in a single experiment, alongside genome-wide RNAs. We use SBARRO to describe synaptic networks formed by diverse mouse brain cell types in vitro, finding that different cell types have presynaptic networks with differences in average size and cell type composition. Patterns of RNA expression suggest that functioning synapses are critical for rabies virus uptake. By tracking individual rabies clones across cells, SBARRO offers new opportunities to map the synaptic organization of neural circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.