ArticleBMC veterinary research2022
Isolation and evaluation of the efficacy of bacteriophages against multidrug-resistant (MDR), methicillin-resistant (MRSA) and biofilm-producing strains of Staphylococcus aureus recovered from bovine mastitis.
Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 36 citations in OpenAlex.
- Identification, antimicrobial resistance, and biofilm-forming capability of bacterial isolates from bovine mastitis in the high-altitude region of Shannan, Tibet.Archives of microbiology · 2026Article
- Prevalence, Antimicrobial Resistance Profile, and Multidrug Resistance of Methicillin-ResistantHealth science reports · 2026Article
- Article
- Phage-antibiotic synergistic effect for treating cutaneous wounds infections caused by MRSA and the assessment of wound healing biomarkers in a rabbit model.Scientific reports · 2026Article
- Phage as control strategy against the foodborne pathogen, Bacillus cereus.Archives of microbiology · 2026Review
- Isolation and characterization of a novelFrontiers in cellular and infection microbiology · 2026Article
- Bacteriophages and Endolysins Used in the Biocontrol ofMicroorganisms · 2025Review
- Bacteriophages as a modern diagnostic tool: innovations, applications and challenges.Molecular biology reports · 2025Review
- In vitro antimicrobial activity of polyhexamethylene guanidine hydrochloride and polyhexamethylene biguanide against microorganisms causing bovine mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Comprehensive Prevention and Control of Mastitis in Dairy Cows: From Etiology to Prevention.Veterinary sciences · 2025Review
- Beware of host immune responses towards bacteriophages potentially impacting phage therapy.Veterinary research · 2025Article
- Review
- Characterization of Newly IsolatedMicroorganisms · 2025Article
- Article
- Antibacterial and antibiofilm activity ofFrontiers in veterinary science · 2025Article
- Sustainable AGP alternatives: a systems approach to non-antibiotic growth regulators standardization, synergistic formulation and environmental safety.Frontiers in veterinary science · 2025Review
- A New Approach for Phage Cocktail Design in the Example of Anti-Mastitis Solution.Pathogens (Basel, Switzerland) · 2024Article
- Immunomodulation in Non-traditional Therapies for Methicillin-resistant Staphylococcus aureus (MRSA) Management.Current microbiology · 2024Review
- Prevalence and Phage-Based Biocontrol of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- When the solution becomes the problem: a review on antimicrobial resistance in dairy cattle.Future microbiology · 2024Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundStaphylococcus aureus (S. aureus) is one of the major causes of bovine mastitis with significant economic losses around the worldwide. The emergence of multidrug-resistant (MDR), methicillin-resistant (MRSA) and biofilm-producing strains of S. aureus challenges the treatment strategies based on the antibiotic application. Today, alternative or combinational treatment options such as bacteriophage application has received much attention. The goal of the present study was to focus on isolation and evaluation of the efficacy of bacteriophages with specific lytic activity against S. aureus strains with low cure rates (MDR, MRSA and biofilm-producing strains).
resultsIn the present study, two phages belonging to the Podoviridae family with specific lytic activity against S. aureus were isolated from the sewage of dairy farms and designated as Staphylococcus phage M8 and Staphylococcus phage B4. Latent period and burst size for Staphylococcus phage M8 (70 min, 72 PFU/cell) and Staphylococcus phage B4 (30 min, 447 PFU/cell) were also defined. Our results revealed the susceptibility of MDR (4/20; 20%), MRSA (4/13; 30.8%) and biofilm-producing (1/10; 10%) strains to Staphylococcus phage M8. Moreover, one biofilm-producing strain (1/10; 10%) was susceptible to Staphylococcus phage B4. Furthermore, both phages kept their lytic activity in milk. They reduced the S. aureus population by about 3 logs in cultured milk after 8 h of incubation.
conclusionIn conclusion, it seems that both phages had the potential to serve as biological control agents alone or in combination with other agents such as antibiotics against infections induced by S. aureus. However, further studies are needed to investigate the efficacy of these phages in vivo.
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