ArticlePlant methods2022
High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Salicylic acid and the unique TGA transcription factor controls plant immunity against Pseudomonas syringae in Marchantia polymorpha.Plant & cell physiology · 2026Article
- Welcome Pathogens: Transient Heat Dampens Immune Responses to Acibenzolar-S-Methyl in Apple Plants.Plant, cell & environment · 2026Article
- Transcriptome and Hormone Analysis Revealed Jasmonic Acid-Mediated Immune Responses of Potato (Antioxidants (Basel, Switzerland) · 2026Article
- Light Quantity Impacts Early Response to Cold and Cold Acclimation in Young Leaves of Arabidopsis.Plant, cell & environment · 2025Article
- Integration of multi-omics data and deep phenotyping provides insights into responses to single and combined abiotic stress in potato.Plant physiology · 2025Article
- Dinor-12-oxo-phytodienoic acid conjugation with amino acids inhibits its phytohormone bioactivity in Marchantia polymorpha.Plant physiology · 2024Article
- Amide conjugates of the jasmonate precursor cis-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses.Plant physiology · 2024Article
- Cell wall integrity modulates HOOKLESS1 and PHYTOCHROME INTERACTING FACTOR4 expression controlling apical hook formation.Plant physiology · 2024Article
- Multi-omics signatures of diverse plant callus cultures.Plant biotechnology (Tokyo, Japan) · 2024Article
- Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry.Plant methods · 2024Article
- In situ separation and visualization of isomeric auxin derivatives in Arabidopsis by ion mobility mass spectrometry imaging.Analytical and bioanalytical chemistry · 2024Article
- Can plant hormonomics be built on simple analysis? A review.Plant methods · 2023Review
- Metabolic profiles of 2-oxindole-3-acetyl-amino acid conjugates differ in various plant species.Frontiers in plant science · 2023Article
- Comparative analysis of wild-type and chloroplast MCU-deficient plants reveals multiple consequences of chloroplast calcium handling under drought stress.Frontiers in plant science · 2023Article
- Glycosylation pathways in auxin homeostasis.Physiologia plantarumReview
- Phyto-Oxylipins as Key Regulators of Plant Resilience to Abiotic and Biotic Stress.Physiologia plantarumReview
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundAcidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an important insight into ongoing physiological processes and is essential for many biological studies performed on plants.
resultsA high-throughput sample preparation method for liquid chromatography-tandem mass spectrometry determination of 25 acidic phytohormones classed as auxins, jasmonates, abscisates and salicylic acid was optimised. The method uses a small amount of plant tissue (less than 10 mg fresh weight) and acidic extraction in 1 mol/L formic acid in 10% aqueous methanol followed by miniaturised purification on reverse phase sorbent accommodated in pipette tips organised in a 3D printed 96-place interface, capable of processing 192 samples in one run. The method was evaluated in terms of process efficiency, recovery and matrix effects as well as establishing validation parameters such as accuracy and precision. The applicability of the method in relation to the amounts of sample collected from distantly related plant species was evaluated and the results for phytohormone profiles are discussed in the context of literature reports.
conclusionThe method developed enables high-throughput profiling of acidic phytohormones with minute amounts of plant material, and it is suitable for large scale interspecies studies.
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