ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2022
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Typing of Treponema pallidum in a Brazilian sample and follow-up of treatment using molecular assays.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Laboratory Evaluation of the Chembio DPP Syphilis Screen & Confirm Point-of-Care Test on Serum and Simulated Blood Samples.Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2024Article
- Advantages and limitations of current diagnostic laboratory approaches in syphilis and congenital syphilis.Expert review of anti-infective therapyReview
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Authors and funding
7 authors.
Funding
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Abstract
Syphilis is caused by the bacterium Treponema pallidum. The diagnosis is based on clinical data and serological analysis; however, the sensitivity and specificity of such tests may vary depending on the type of test and stage of the infection. In order to overcome this premise, this study utilized the polymerase chain reaction (PCR) for the detection of T. pallidum DNA in whole blood samples of patients with syphilis. The blood samples from patients with or without symptoms of syphilis, but with positive results in enzyme-linked immunosorbent assay (ELISA), were included in this study. A venereal disease research laboratory (VDRL) test was performed for all collected sera samples. For PCR, the T. pallidum DNA was extracted from the collected blood samples and a specific primer set was designed to amplify 131 nucleotides of polA (Tp0105). The specificity of the primers was evaluated with the DNA of 17 different pathogens. From a total of 314 blood samples reactive in ELISA, 58.2% (183/314) of the samples were reactive in the VDRL test. In the PCR, 54% (168/314) of the ELISA-reactive samples were positive. In both tests (VDRL and PCR) 104 samples were positive. Of 104 positive samples for both tests, 71 were at the latent stage. Based on these results, it can be concluded that PCR with the designed set of primers can be utilized as a diagnostic method for T. pallidum detection in blood samples of patients with syphilis, especially those with latent infection. In addition, it can be utilized as a supplement for serological methods to improve the diagnosis of syphilis.
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