ArticleACS nano2022
Particle Localization Using Local Gradients and Its Application to Nanometer Stabilization of a Microscope.
Article in ACS nano, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- Open-source 3D active sample stabilization for fluorescence microscopy.Biophysical reports · 2025Article
- A Compact Prism-Based Microscope for Highly Sensitive Measurements in Fluid Biopsy.Journal of biophotonics · 2025Article
- A new method to experimentally quantify dynamics of initial protein-protein interactions.Communications biology · 2024Article
- Resolution enhancement with deblurring by pixel reassignment (DPR).bioRxiv : the preprint server for biology · 2023Article
- Resolution enhancement with deblurring by pixel reassignment.Advanced photonicsArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Particle localization plays a fundamental role in advanced biological techniques such as single-molecule tracking, superresolution microscopy, and manipulation by optical and magnetic tweezers. Such techniques require fast and accurate particle localization algorithms as well as nanometer-scale stability of the microscope. Here, we present a universal method for three-dimensional localization of single labeled and unlabeled particles based on local gradient calculation of particle images. The method outperforms state-of-the-art localization techniques in high-noise conditions, and it is capable of 3D nanometer accuracy localization of nano- and microparticles with sub-millisecond calculation time. By localizing a fixed particle as fiducial mark and running a feedback loop, we demonstrate its applicability for active drift correction in sensitive nanomechanical measurements such as optical trapping and superresolution imaging. A multiplatform open software package comprising a set of tools for local gradient calculation in brightfield, darkfield, and fluorescence microscopy is shared for ready use by the scientific community.
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Registered trials
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