Evidence map›Paper›PMID 36383329›Full record

ArticleAntonie van Leeuwenhoek2023

FtsZ phosphorylation pleiotropically affects Z-ladder formation, antibiotic production, and morphogenesis in Streptomyces coelicolor.

Paula Yagüe, Joost Willemse, Xiansha Xiao, Le Zhang, Angel Manteca, Gilles P van Wezel

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antonie van Leeuwenhoek, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Paula YagüeDepartment of Molecular Biotechnology, Institute of Biology Leiden, Leiden University, PO Box 9505, 2300 AB, Leiden, The Netherlands. yaguepaula@uniovi.es.ORCID http://orcid.org/0000-0003-1526-3121
Joost WillemseDepartment of Molecular Biotechnology, Institute of Biology Leiden, Leiden University, PO Box 9505, 2300 AB, Leiden, The Netherlands.ORCID http://orcid.org/0000-0001-9312-6021
Xiansha XiaoDepartment of Molecular Biotechnology, Institute of Biology Leiden, Leiden University, PO Box 9505, 2300 AB, Leiden, The Netherlands.ORCID http://orcid.org/0000-0002-7617-0212
Le ZhangDepartment of Molecular Biotechnology, Institute of Biology Leiden, Leiden University, PO Box 9505, 2300 AB, Leiden, The Netherlands.ORCID http://orcid.org/0000-0002-8677-7595
Angel MantecaDepartamento de Biología Funcional e IUOPA, Área de Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain.ORCID http://orcid.org/0000-0001-8001-8988
Gilles P van WezelDepartment of Molecular Biotechnology, Institute of Biology Leiden, Leiden University, PO Box 9505, 2300 AB, Leiden, The Netherlands.ORCID http://orcid.org/0000-0003-0341-1561
Leiden University · NLUniversidad de Oviedo · ES

Funding

Ministerio de Ciencia, Innovación y Universidades RTI2018-093978-B-I00
6 · The paper itself

Abstract

The GTPase FtsZ forms the cell division scaffold in bacteria, which mediates the recruitment of the other components of the divisome. Streptomycetes undergo two different forms of cell division. Septa without detectable peptidoglycan divide the highly compartmentalised young hyphae during early vegetative growth, and cross-walls are formed that dissect the hyphae into long multinucleoid compartments in the substrate mycelium, while ladders of septa are formed in the aerial hyphae that lead to chains of uninucleoid spores. In a previous study, we analysed the phosphoproteome of Streptomyces coelicolor and showed that FtsZ is phosphorylated at Ser 317 and Ser389. Substituting Ser-Ser for either Glu-Glu (mimicking phosphorylation) or Ala-Ala (mimicking non-phosphorylation) hinted at changes in antibiotic production. Here we analyse development, colony morphology, spore resistance, and antibiotic production in FtsZ knockout mutants expressing FtsZ alleles mimicking Ser319 and Ser387 phosphorylation and non-phosphorylation: AA (no phosphorylation), AE, EA (mixed), and EE (double phosphorylation). The FtsZ-eGFP AE, EA and EE alleles were not able to form observable FtsZ-eGFP ladders when they were expressed in the S. coelicolor wild-type strain, whereas the AA allele could form apparently normal eGFP Z-ladders. The FtsZ mutant expressing the FtsZ EE or EA or AE alleles is able to sporulate indicating that the mutant alleles are able to form functional Z-rings leading to sporulation when the wild-type FtsZ gene is absent. The four mutants were pleiotropically affected in colony morphogenesis, antibiotic production, substrate mycelium differentiation and sporulation (sporulation timing and spore resistance) which may be an indirect result of the effect in sporulation Z-ladder formation. Each mutant showed a distinctive phenotype in antibiotic production, single colony morphology, and sporulation (sporulation timing and spore resistance) indicating that the different FtsZ phosphomimetic alleles led to different phenotypes. Taken together, our data provide evidence for a pleiotropic effect of FtsZ phosphorylation in colony morphology, antibiotic production, and sporulation.

Indexed as

StreptomycesStreptomyces coelicolorAnti-Bacterial AgentsBacterial ProteinsCell WallSpores, BacterialAnti-Bacterial AgentsBacterial ProteinsCell divisionDifferentiationSerine-phosphorylationSporulationStreptomyces

Identifiers

PMID36383329
PMCPMC9823044
OpenAlexW4309164579

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.