ArticleIranian journal of biotechnology2022
Enhanced Acetate Tolerance and Recombinant Protein Accumulation in
Article in Iranian journal of biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 1 citations in OpenAlex.
- Gene Editing and AI: Revolutionizing Biotechnology for a Sustainable Future.Iranian journal of biotechnology · 2026Article
- Application of Acetate as a Substrate for the Production of Value-Added Chemicals inMicroorganisms · 2024Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Background: In Objectives: We examined whether the heterologous expression of a eukaryotic heat shock protein (Hsp) can confer tolerance to acetate in Materials and Methods: Transgenic cell lines (TCLs) heterologously expressing a small heat shock protein (sHsp) from carrot ( Results: TCLs constitutively expressing DcHsp17.7 showed improved growth, cell density, and cell viability under the stress conditions of heat, acetate, and alkaline compared to an empty vector control line. In acetate stress conditions, TCL1 accumulated more cellular proteins (approximately 130%) than the control. The recombinant ADH accumulated to a higher level in TCL1 (2.2-fold at 16 °C) than the control. The addition of acetate reduced the recombinant ADH level by 70% in the control when compared with the absence of acetate. In contrast, recombinant ADH accumulation was not affected by acetate in TCL1. In the presence of acetate, TCL1 accumulated 6.4-fold more recombinant ADH than did the control. Furthermore, recombinant ADH produced in TCL1 showed 1.5-fold higher enzyme activity than that produced in the control in the presence or absence of acetate. Conclusion: Our study showed that heterologously expressed DcHsp17.7 from carrot can alleviate the negative effects of acetate on
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