Evidence map›Paper›PMID 36381150›Full record

ArticleIUCrJ2022

Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.

Kara A Zielinski, Andreas Prester, Hina Andaleeb, Soi Bui, Oleksandr Yefanov, Lucrezia Catapano, Alessandra Henkel, Max O Wiedorn, Olga Lorbeer, Eva Crosas and 14 more

Open access · goldAbstract read
In one paragraph

Article in IUCrJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 36 citations in OpenAlex.

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  15. Journal of applied crystallography · 2024
    Article
  16. Article
  17. A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024
    Review
  18. Article
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors at 5 institutions in 3 countries.

Kara A ZielinskiCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-9907-5058
Andreas PresterInstitute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Hina AndaleebInstitute of Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany.ORCID https://orcid.org/0000-0002-1084-9652
Soi BuiRandall Centre of Cell and Molecular Biophysics, King's College London, United Kingdom.
Oleksandr YefanovCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0001-8676-0091
Lucrezia CatapanoRandall Centre of Cell and Molecular Biophysics, King's College London, United Kingdom.
Alessandra HenkelCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Max O WiedornCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Olga LorbeerDeutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Eva CrosasDeutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Jan MeyerDeutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Valerio MarianiCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Martin DomarackyCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Thomas A WhiteDeutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-8407-0270
Holger FleckensteinCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-7216-6494
Iosifina SarrouCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-6963-5341
Nadine WernerInstitute of Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany.
Christian BetzelInstitute of Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany.
Holger RohdeInstitute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Martin AepfelbacherInstitute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Henry N ChapmanCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-4655-1743
Markus PerbandtInstitute of Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany.
Roberto A SteinerRandall Centre of Cell and Molecular Biophysics, King's College London, United Kingdom.ORCID https://orcid.org/0000-0001-7084-9745
Dominik OberthuerCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0002-0894-9590
Universität Hamburg · DEDeutsches Elektronen-Synchrotron DESY · DEKing's College London · GBMRC Laboratory of Molecular Biology · GBUniversity of Padua · IT

Funding

Medical Research Council MC_UP_A025_1012
6 · The paper itself

Abstract

Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (

Indexed as

A. flavus urate oxidaseCFEL TapeDriveK. pneumoniae CTX-M-14N. haematococca GH11 xylanasepartial reflectionsprotein structuresroom temperaturesample deliveryserial crystallography

Identifiers

PMID36381150
PMCPMC9634612
OpenAlexW4307925887

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.