Evidence map›Paper›PMID 36380954›Full record

ArticleRSC advances2022

Nano-optical method for transforming a single yeast cell using exogenous genes.

Yao-Xiong Huang, Ji-Wang Yang, Zhuo Wang

Open access · goldAbstract read
In one paragraph

Article in RSC advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yao-Xiong HuangDepartment of Biomedical Engineering, Ji Nan University Guangzhou China 510632 tyxhuang@jnu.edu.cn +86-20-85223742 +86-20-85223742.ORCID https://orcid.org/0000-0002-9376-053X
Ji-Wang YangDepartment of Biomedical Engineering, Ji Nan University Guangzhou China 510632 tyxhuang@jnu.edu.cn +86-20-85223742 +86-20-85223742.
Zhuo WangDepartment of Biomedical Engineering, Ji Nan University Guangzhou China 510632 tyxhuang@jnu.edu.cn +86-20-85223742 +86-20-85223742.ORCID https://orcid.org/0000-0002-0190-2589

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report a highly efficient nano-optical method for transforming a single yeast cell using exogenous genes. It used laser tweezers or micromanipulators to immobilize the cell immersed in a DNA solution and created a transient nano-sized hole on its cell wall concurrently with laser scissors to deliver nano moles of DNA into the cell. With this method, one can directly transfer the naked DNA of exogenous genes into yeast cells for transformation. We successfully transformed

Identifiers

PMID36380954
PMCPMC9639168
OpenAlexW4309242938

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.