ArticleACS ES&T water2022
Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater.
Article in ACS ES&T water, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 2 syntheses or guidelines pooled it, 129 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Multiplexed detection of febrile infections using CARMEN.Nature communications · 2025Trial
- Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay.MethodsX · 2026Article
- Laboratory Assessment of Wastewater as Surveillance Tool for West Nile Virus, United States.Emerging infectious diseases · 2026Article
- Improving Inference from Reported Concentrations in Environmental Surveillance by Modeling the Statistical Features of Digital PCR.ACS ES&T water · 2026Article
- Bio-Rad and QIAGEN digital PCR platforms provide equivalent quantification for wastewater-based SARS-CoV-2 surveillance.Applied and environmental microbiology · 2026Article
- Could the load of carbapenemase genes in hospital wastewater be a proxy for emerging resistance to carbapenems in humans?Antimicrobial resistance and infection control · 2026Article
- Wastewater genomic surveillance identifies the emergence of the SARS-CoV-2 JN.1 lineage in urban settings of Gujarat.Frontiers in microbiology · 2026Article
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- Spatially Informed Wastewater Differentiation among Locations during an Ongoing Measles Outbreak in Texas, USA.ACS environmental Au · 2025Article
- Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples.BMC veterinary research · 2025Article
- Simplifying SARS-CoV-2 wastewater-based surveillance using an automated FDA EUA assay.Microbiology spectrum · 2025Article
- The use of wastewater surveillance to estimate SARS-CoV-2 fecal viral shedding pattern and identify time periods with intensified transmission.BMC public health · 2025Article
- Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental Surveillance.ACS synthetic biology · 2025Article
- The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.Microorganisms · 2025Article
- Article
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- Establishing methods to monitor H5N1 influenza virus in dairy cattle milk.medRxiv : the preprint server for health sciences · 2024Article
- Effective Inhibitor Removal from Wastewater Samples Increases Sensitivity of RT-dPCR and Sequencing Analyses and Enhances the Stability of Wastewater-Based Surveillance.Microorganisms · 2024Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low-prevalence COVID-19 locations in Queensland, Australia, using CDC N1 and CDC N2 assays. The assay limit of detection (ALOD), PCR inhibition rates, and performance characteristics of each assay, along with the positivity rates with the RT-qPCR and RT-dPCR platforms, were evaluated by seeding known concentrations of exogenous SARS-CoV-2 in wastewater. The ALODs using RT-dPCR were approximately 2-5 times lower than those using RT-qPCR. During sample processing, the endogenous (
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.