Evidence map›Paper›PMID 36380259›Full record

ArticleMolecular biotechnology2023

Sequence-Specific Gene Silencing of acrA in the Multi-drug Efflux System AcrAB Induces Sensitivity in Drug-Resistant Klebsiella pneumoniae.

Priyanka Ashwath, Vijaya Kumar Deekshit, Anusha Rohit, Praveen Rai, Vankadari Aditya, Nishith Babu, Indrani Karunasagar, Akhila Dharnappa Sannejal

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Priyanka AshwathNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Vijaya Kumar DeekshitNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Anusha RohitNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Praveen RaiNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Vankadari AdityaNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Nishith BabuNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Indrani KarunasagarNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India.
Akhila Dharnappa SannejalNitte University Centre for Science Education and Research, Nitte (Deemed to be University), Deralakatte, Mangaluru, 575018, India. akhila@nitte.edu.in.ORCID http://orcid.org/0000-0002-2494-225X
Nitte University · IN

Funding

Nitte (Deemed to be) University NUFR1/2018/10/17
6 · The paper itself

Abstract

Multi-drug efflux is one of the resistant determinants in Klebsiella pneumoniae that are encountered in a broad range of clinically relevant antimicrobial agents. An alternative method to strategically induce sensitivity in drug-resistant K. pneumoniae and improve the efficacy of the existing antibiotics is the need of the hour. Hence, an antisense RNA was designed against the acrA gene of the AcrAB-TolC efflux system in a drug-resistant isolate of K. pneumoniae obtained from a blood culture. Minimum inhibitory concentration by E test demonstrated that the antisense RNA could significantly increase the susceptibility of previously resistant K. pneumoniae toward ciprofloxacin (CIP) and co-trimoxazole. Real-time PCR determined the ability of the antisense RNA to inhibit the expression of the acrA-mRNA. The wild-type K. pneumoniae showed increased growth in the presence of CIP, while, under the same condition, the growth of the antisense RNA-treated K. pneumoniae was inhibited up till 12 h. In the presence of co-trimoxazole, delayed growth rate of the antisense RNA-treated K. pneumoniae was seen, in comparison to that of the wild-type K. pneumoniae and also a fourfold reduction was noted in the expression of the efflux gene acrA. Our results underscore the potential of the acrA antisense RNA as an alternative therapeutic against multi-drug-resistant K. pneumoniae.

Indexed as

Klebsiella InfectionsKlebsiella pneumoniaeAnti-Bacterial AgentsBacterial ProteinsHumansMicrobial Sensitivity TestsTrimethoprim, Sulfamethoxazole Drug CombinationAnti-Bacterial AgentsBacterial ProteinsTrimethoprim, Sulfamethoxazole Drug CombinationAntisense RNAGene knockdownKlebsiella pneumoniaeMulti-drug efflux

Identifiers

PMID36380259
OpenAlexW4309165979

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.