Evidence map›Paper›PMID 36380160›Full record

ArticleActa parasitologica2023

Levels of Genetic Variants Among Symptomatic Blastocystis Subtypes and their Relationship to Mucosal Immune Surveillance in the Precancerous Colons of Experimentally Infected Rats.

Eman M Hussein, Muhammad A A Muhammad, Abdalla M Hussein, Sherine M Elzagawy, Wafaa M Zaki, Ashraf G Temsah, Mohamed S Badr, Maha M Alabbassy

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In one paragraph

Article in Acta parasitologica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Eman M HusseinMedical Parasitology Department, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt. ehussein@med.suez.edu.eg.ORCID http://orcid.org/0000-0003-3362-2925
Muhammad A A MuhammadPathology Department, Faculty of Medicine, Suez Canal, University, Ismailia, 41522, Egypt.
Abdalla M HusseinBio-Physics Department, Faculty of Science, Al-Azhar University, Cairo, 11652, Egypt.
Sherine M ElzagawyMedical Parasitology Department, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Wafaa M ZakiMedical Parasitology Department, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Ashraf G TemsahMedical Parasitology Department, Faculty of Medicine, Damietta Branch, AL Azhar University, Damietta, Egypt.
Mohamed S BadrMedical Genetic Centre, Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Maha M AlabbassyMedical Parasitology Department, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Suez Canal University · EGAin Shams University · EGAl-Azhar University · EGDamietta University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe relationship between the genetic diversity of Blastocystis and immune surveillance in precancerous colons with blastocystosis is still under investigation. This study aimed to identify the genetic Blastocystis variants among 54 symptomatic human isolates and their relationship to mucosal immune surveillance in the precancerous polyps of experimentally infected rats.

methodsPolymerase chain reaction and high-resolution melting (PCR/HRM) curves discriminated human symptomatic Blastocystis isolates into subtypes (STs)/intrasubtypes, which were orally administered to rats to induce experimental infection. Then, the mucosal immune responses of the infected colons were evaluated in relation to polyp formation through immunostaining to identify mucus MUC2 and determine mucosal immune cell (goblet, lymphocyte and mast) counts, secretory IgA levels and parasitic intestinal invasion.

resultsST1, ST3, and ST4 were found in 18.5% (10/54), 54.7% (29/54), and 27.8% (15/54) of the samples, respectively. Then, the HRM curve discriminated ST3 into the wild, mutant, and heterozygous [17/54 (31.5%), 5/54 (9.3%), and 7/54 (12.9%)] intrasubtypes. ST1 and ST4 had no genetic variations. Precancerous polyps were detected in the colons of 40.5% of the infected rats. ST1 constituted 14.7% of these cases, while the wild, mutant, and heterozygous intrasubtypes of ST3 showed polyps in 12.9%, 5.5%, and 5.5% of cases, respectively. Only 1.9% of the polyps were related to ST4. MUC2 showed weak immunostaining in 44.5% of the infected colons, and 38.9% were polyp inducers. Low goblet cell numbers and high interepithelial lymphocyte counts were significantly associated with polyp formation, particularly with ST1 and wild ST3. Among the polyp inducers, high numbers of mast cells were detected in wild ST3 and ST4, while a low number was found with heterozygous ST3. The level of secretory IgA was low in polyp-inducing STs. Most of the results were statistically significant.

conclusionImmunosurveillance showed a potential relationship between ST1 and the ST3 intrasubtypes and precancerous polyps. This relationship may provide insight into the prevention and/or development of new immunotherapeutic strategies to combat colorectal cancer.

Indexed as

BlastocystisBlastocystis InfectionsPrecancerous ConditionsAnimalsDNA, ProtozoanFecesHumansPhylogenyRatsDNA, ProtozoanBlastocystisImmune surveillanceIntrasubtypeMUC2PolypsPrecancerous

Identifiers

PMID36380160
PMCPMC10011339
OpenAlexW4309070829

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read111
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.