ArticleCurrent biology : CB2022
The nematode Oscheius tipulae as a genetic model for programmed DNA elimination.
Article in Current biology : CB, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 24 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Article
- Programmed DNA elimination was present in the last common ancestor ofbioRxiv : the preprint server for biology · 2026Article
- The tiny germline chromosomes of Paramecium aurelia have an exceptionally high recombination rate and are capped by a new class of Helitrons.BMC biology · 2026Article
- A sequence motif for DNA double-strand break and telomere healing during programmed DNA elimination.bioRxiv : the preprint server for biology · 2026Article
- Blue light stimulated-autofluorescence green flash of lysosome-related organelles in the intestinal cells of nematodes.G3 (Bethesda, Md.) · 2025Article
- Parallel Evolution of X Chromosome-Specific Structural Maintenance of Chromosomes Complexes in Two Nematode Lineages.Molecular biology and evolution · 2025Article
- Blue light stimulated-autofluorescence green flash of lysosome-related organelles in the intestinal cells of nematodes.bioRxiv : the preprint server for biology · 2025Article
- How and when organisms edit their own genomes.Nature genetics · 2025Review
- Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination.Biochemical Society transactions · 2025Review
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.Nucleic acids research · 2024Article
- Chromosome fusion and programmed DNA elimination shape karyotypes of nematodes.Current biology : CB · 2024Article
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.bioRxiv : the preprint server for biology · 2024Article
- Environmental radiation exposure at Chornobyl has not systematically affected the genomes or chemical mutagen tolerance phenotypes of local worms.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chromosome fusion and programmed DNA elimination shape karyotypes of parasitic nematodes.bioRxiv : the preprint server for biology · 2023Article
- Programmed DNA elimination in the parasitic nematode Ascaris.PLoS pathogens · 2023Review
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10 authors at 1 institution in 1 country.
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Abstract
Programmed DNA elimination (PDE) is a notable exception to the paradigm of genome integrity. In metazoa, PDE often occurs coincident with germline to somatic cell differentiation. During PDE, portions of genomic DNA are lost, resulting in reduced somatic genomes. Prior studies have described the sequences lost, as well as chromosome behavior, during metazoan PDE. However, a system for studying the mechanisms and consequences of PDE in metazoa is lacking. Here, we present a functional and genetic model for PDE in the free-living Rhabditidae nematode Oscheius tipulae, a family that also includes Caenorhabditis elegans. O. tipulae was recently suggested to eliminate DNA. Using staged embryos and DNA FISH, we showed that O. tipulae PDE occurs during embryogenesis at the 8-16 cell stages. We identified a conserved motif, named Sequence For Elimination (SFE), for all 12 break sites on the six chromosomes at the junctions of retained and eliminated DNA. SFE mutants exhibited a "fail-to-eliminate" phenotype only at the modified sites. END-seq revealed that breaks can occur at multiple positions within the SFE, with extensive end resection followed by telomere addition to both retained and eliminated ends. We identified many functional SFEs at the chromosome ends through END-seq in the wild-type embryos, genome sequencing of SFE mutants, and comparative genomics of 23 wild isolates. We suggest that these alternative SFEs provide flexibility in the sequences eliminated and a fail-safe mechanism for PDE. These studies establish O. tipulae as a new, attractive model for studying the mechanisms and consequences of PDE in a metazoan.
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