Evidence map›Paper›PMID 36378443›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

2D-DIGE Analysis of Liver Disease in Mice.

Aïsha Callebaut, Rita Derua, Lut Overbergh, Veerle Janssens

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 64% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Aïsha CallebautLaboratory for Clinical and Experimental Endocrinology, Department of Chronic Diseases & Metabolism, University of Leuven (KU Leuven), Leuven, Belgium.
Rita DeruaLaboratory of Protein Phosphorylation & Proteomics, Department of Cellular & Molecular Medicine, University of Leuven (KU Leuven), Leuven, Belgium.
Lut OverberghLaboratory for Clinical and Experimental Endocrinology, Department of Chronic Diseases & Metabolism, University of Leuven (KU Leuven), Leuven, Belgium.
Veerle JanssensLaboratory of Protein Phosphorylation & Proteomics, Department of Cellular & Molecular Medicine, University of Leuven (KU Leuven), Leuven, Belgium. veerle.janssens@kuleuven.be.
KU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatocellular carcinoma (HCC) is the major type of primary liver cancer. In this chapter, we describe our routine two-dimensional difference gel electrophoresis (2D-DIGE) workflow for analysis of mouse liver tissue in physiological conditions, as well as of mouse HCC. 2D-DIGE still constitutes a valuable comparative proteomics technique, not only providing information on global protein expression in a sample but also on potential posttranslational protein modifications, occurrence of protein degradation fragments, and the existence of protein isoforms. Thus, 2D-DIGE analysis provides highly complementary data to non-gel-based shotgun mass spectrometry (MS) methods (e.g., liquid chromatography (LC)-MS/MS)-allowing, for example, identification of novel protein biomarkers for HCC or increasing insights into the molecular mechanisms underlying hepatocarcinogenesis.

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsAnimalsElectrophoresis, Gel, Two-DimensionalMiceProtein IsoformsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTandem Mass SpectrometryTwo-Dimensional Difference Gel ElectrophoresisProtein IsoformsComparative proteomicsHepatocellular carcinoma (HCC)Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)Liver cancerMatrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS/MS)Mouse tissueTwo-dimensional difference gel electrophoresis (2D-DIGE)

Identifiers

PMID36378443
OpenAlexW4309199933

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.