Evidence map›Paper›PMID 36378343›Full record

ArticleCellular and molecular life sciences : CMLS2022

CFTR modulates aquaporin-mediated glycerol permeability in mouse Sertoli cells.

João C Ribeiro, Raquel L Bernardino, David F Carrageta, Graça Soveral, Giuseppe Calamita, Marco G Alves, Pedro F Oliveira

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

João C RibeiroDepartment of Anatomy, Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Raquel L BernardinoDepartment of Anatomy, Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
David F CarragetaDepartment of Anatomy, Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Graça SoveralResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal.
Giuseppe CalamitaDepartment of Biosciences, Biotechnologies and Environment, University of Bari "Aldo Moro", Bari, Italy.
Marco G AlvesDepartment of Anatomy, Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Pedro F OliveiraLAQV-REQUIMTE and Department of Chemistry, University of Aveiro, Aveiro, Portugal. p.foliveira@ua.pt.ORCID http://orcid.org/0000-0002-4989-5699
Universidade do Porto · PTRede de Química e Tecnologia · PTUniversity of Bari Aldo Moro · ITUniversity of Lisbon · PT

Funding

Fundação para a Ciência e a Tecnologia CEECINST/00026/2018Fundação para a Ciência e a Tecnologia UI/BD/150749/2020Fundação para a Ciência e a Tecnologia UIDB/00215/2020Fundação para a Ciência e a Tecnologia UIDB/50006/2020Fundação para a Ciência e a Tecnologia UIDP/00215/2020ITR - Laboratory for Integrative and Translational Research in Population Health LA/P/0064/2020
6 · The paper itself

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that is crucial for fluid homeodynamics throughout the male reproductive tract. Previous evidence shed light on a potential molecular partnership between this channel and aquaporins (AQPs). Herein, we explore the role of CFTR on AQPs-mediated glycerol permeability in mouse Sertoli cells (mSCs). We were able to identify the expression of CFTR, AQP3, AQP7, and AQP9 in mSCs by RT-PCR, Western blot, and immunofluorescence techniques. Cells were then treated with CFTRinh-172, a specific CFTR inhibitor, and its glycerol permeability was evaluated by stopped-flow light scattering. We observed that CFTR inhibition decreased glycerol permeability in mSCs by 30.6% when compared to the control group. A DUOLINK proximity ligation assay was used to evaluate the endogenous protein-protein interactions between CFTR and the various aquaglyceroporins we identified. We positively detected that CFTR is in close proximity with AQP3, AQP7, and AQP9 and that, through a possible physical interaction, CFTR can modulate AQP-mediated glycerol permeability in mSCs. As glycerol is essential for the control of the blood-testis barrier and elevated concentration in testis results in the disruption of spermatogenesis, we suggest that the malfunction of CFTR and the consequent alteration in glycerol permeability is a potential link between male infertility and cystic fibrosis.

Indexed as

AquaporinsGlycerolAnimalsCystic Fibrosis Transmembrane Conductance RegulatorMaleMicePermeabilitySertoli CellsAquaporinsCftr protein, mouseCystic Fibrosis Transmembrane Conductance RegulatorGlycerolAquaglyceroporinCFTRGlycerolMale infertilitySertoli cell

Identifiers

PMID36378343
PMCPMC11803011
OpenAlexW4309199549

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.