ArticleScientific reports2022
Osteogenic differentiation and proliferation potentials of human bone marrow and umbilical cord-derived mesenchymal stem cells on the 3D-printed hydroxyapatite scaffolds.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 2 of them syntheses that pooled it.
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Who cites it
44 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Clinical and research applications of synthetic bone substitutes in equine veterinary medicine: A systematic review.Equine veterinary journal · 2026Pooled it
- Influence of the addition of nanohydroxyapatite to scaffolds on proliferation and differentiation of human mesenchymal stem cells: a systematic review of in vitro studies.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2024Pooled it
- Enhanced Osteoinduction, Rheological and Mechanical Performance of 3D-Printed Methylcellulose-Gelatin-Hydroxyapatite Scaffolds.Journal of functional biomaterials · 2026Article
- Bone organoids and mitochondrial reprogramming.Journal of orthopaedic translation · 2026Review
- Preliminary Formulation-Dependent Angiogenesis-Related and Early Osteogenic Responses to Three-Dimensional Bioprinted Hydroxyapatite-Acrylated Palm Olein Scaffolds: An In Vitro Study.International journal of molecular sciences · 2026Article
- Innovative Hydroxyapatite-Hydrogel Composites for Cartilage Regeneration.Gels (Basel, Switzerland) · 2026Review
- Aqueous Two-Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells.Advanced healthcare materials · 2026Article
- Human Umbilical Cord-Derived Mesenchymal Stem Cells-Involved Strategies: Advancing Tendon Injuries Therapy Towards Clinical Translation.Stem cell reviews and reports · 2026Review
- Advancements in bone organoids: perspectives on construction methodologies and application strategies.Journal of advanced research · 2026Review
- Comprehensive Toxicological Evaluation of 3D-Printed Hydroxyapatite (3DPHA) for Bone Grafting Applications.International journal of molecular sciences · 2026Article
- The Spatiotemporal Heterogeneity of Tumor-Associated Stromal Cells: Reprogramming Plasticity to Unlock Precision Cancer Immunotherapy.Cancer communications (London, England) · 2026Review
- Combination therapy with bone marrow-derived mesenchymal stem cell transplantation and propolis improves streptozotocin-induced kidney injury in diabetic rats.Avicenna journal of phytomedicine · 2026Article
- Bioprinted Constructs in the Regulatory Landscape: Current State and Future Perspectives.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Bone regenerative efficacy of binder-jet fabricated hydroxyapatite granules with and without biomimetic octacalcium phosphate-coated modification in a rat critical-sized calvarial defect model.Regenerative biomaterials · 2026Article
- Mesenchymal Stem Cell Based-Regenerative Therapy in Atherosclerosis: an Updated Review.Current cardiology reports · 2025Review
- The Impact of Post-Printing Hydration in NaCl Solution on the Properties of Binder Jet 3D-Printed Calcium Sulfate and Its Converted Hydroxyapatite.Journal of functional biomaterials · 2025Article
- Osteogenic Potential of Various Premixed Hydraulic Calcium Silicate-Based Sealers on Human Bone Marrow Stem Cells.Materials (Basel, Switzerland) · 2025Article
- From Biomechanics to Bioinnovation: Emerging Applications of Piezoelectric Materials and Phenomena in Dentistry.Biomedicines · 2025Review
- [Influence of porosity and Young's modulus of gelatin methacrylate anhydride hydrogels on the biological behavior of mouse bone marrow mesenchymal stem cells].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2025Article
- Utilization of Stem Cells in Medicine: A Narrative Review.International journal of molecular sciences · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) are a promising candidate for bone repair. However, the maintenance of MSCs injected into the bone injury site remains inefficient. A potential approach is to develop a bone-liked platform that incorporates MSCs into a biocompatible 3D scaffold to facilitate bone grafting into the desired location. Bone tissue engineering is a multistep process that requires optimizing several variables, including the source of cells, osteogenic stimulation factors, and scaffold properties. This study aims to evaluate the proliferation and osteogenic differentiation potentials of MSCs cultured on 2 types of 3D-printed hydroxyapatite, including a 3D-printed HA and biomimetic calcium phosphate-coated 3D-printed HA. MSCs from bone marrow (BM-MSCs) and umbilical cord (UC-MSCs) were cultured on the 3D-printed HA and coated 3D-printed HA. Scanning electron microscopy and immunofluorescence staining were used to examine the characteristics and the attachment of MSCs to the scaffolds. Additionally, the cell proliferation was monitored, and the ability of cells to differentiate into osteoblast was assessed using alkaline phosphatase (ALP) activity and osteogenic gene expression. The BM-MSCs and UC-MSCs attached to a plastic culture plate with a spindle-shaped morphology exhibited an immunophenotype consistent with the characteristics of MSCs. Both MSC types could attach and survive on the 3D-printed HA and coated 3D-printed HA scaffolds. The MSCs cultured on these scaffolds displayed sufficient osteoblastic differentiation capacity, as evidenced by increased ALP activity and the expression of osteogenic genes and proteins compared to the control. Interestingly, MSCs grown on coated 3D-printed HA exhibited a higher ALP activity and osteogenic gene expression than those cultured on the 3D-printed HA. The finding indicated that BM-MSCs and UC-MSCs cultured on the 3D-printed HA and coated 3D-printed HA scaffolds could proliferate and differentiate into osteoblasts. Thus, the HA scaffolds could provide a suitable and favorable environment for the 3D culture of MSCs in bone tissue engineering. Additionally, biomimetic coating with octacalcium phosphate may improve the biocompatibility of the bone regeneration scaffold.
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