Evidence map›Paper›PMID 36375085›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

Super-resolution re-scan second harmonic generation microscopy.

Stefan G Stanciu, Radu Hristu, George A Stanciu, Denis E Tranca, Lucian Eftimie, Adrian Dumitru, Mariana Costache, Harald A Stenmark, Harm Manders, Amit Cherian and 2 more

Open access · hybridAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
7.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Stefan G StanciuCenter for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.
Radu HristuCenter for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.
George A StanciuCenter for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.
Denis E TrancaCenter for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.
Lucian EftimiePathology Department, Emergency Military Hospital, 010825 Bucharest, Romania.ORCID 0000-0003-2014-2543
Adrian DumitruDepartment of Pathology, Bucharest Emergency University Hospital, 050098 Bucharest, Romania.
Mariana CostacheDepartment of Pathology, Bucharest Emergency University Hospital, 050098 Bucharest, Romania.ORCID 0000-0002-3919-862X
Harald A StenmarkDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, 0379 Oslo, Norway.ORCID 0000-0002-1971-4252
Harm MandersConfocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands.
Amit CherianConfocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands.
Mariliis Tark-DameConfocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands.
Erik M M MandersConfocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands.
Universitatea Națională de Știință și Tehnologie Politehnica București · ROClinical Emergency Hospital Bucharest · ROBucharest Emergency University HospitalOslo University Hospital · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.

Indexed as

Second Harmonic Generation MicroscopyCollagenMicroscopy, FluorescencePhotonsRadionuclide ImagingCollagensecond harmonic generationsuper-resolved optical imagingtwo-photon excited fluorescence

Identifiers

PMID36375085
PMCPMC9704717
OpenAlexW4308948337

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.