ArticleProceedings of the National Academy of Sciences of the United States of America2022
Chemical imaging of cellular ultrastructure by null-deflection infrared spectroscopic measurements.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 24 citations in OpenAlex.
- Nanoscale mapping of composition and orientation in electrospun polymeric nanofibers loaded with carbon atomic wires.Scientific reports · 2026Article
- Infrared Nano-Spectroscopy of Single Cell Sections at the Ultrastructural Level.Nanophotonics (Berlin, Germany) · 2026Article
- Assembly of high-performance van der Waals devices using commercial polyvinyl chloride films.Scientific reports · 2026Article
- Machine learning-integrated electrochemical sensing of ciprofloxacin for digital point-of-care therapeutic drug monitoring.Mikrochimica acta · 2025Article
- Nanoscale Analysis beyond Imaging by Atomic Force Microscopy: Molecular Perspectives on Oncology and Neurodegeneration.Small science · 2025Review
- Monitoring Molecular Uptake and Cancer Cells' Response by Development of Quantitative Drug Derivative Probes for Chemical Imaging.Analytical chemistry · 2025Article
- Visualizing Cell Structures with Minecraft.Biophysicist (Rockville, Md.) · 2025Article
- Electrically modulated photothermal force microscopy for revealing molecular conformation changes during polarization switching at the nanoscale.Nature communications · 2025Article
- An analytical model of label-free nanoscale chemical imaging reveals avenues toward improved spatial resolution and sensitivity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- An end-to-end approach for single-cell infrared absorption spectroscopy of bacterial inclusion bodies: from AFM-IR measurement to data interpretation of large sample sets.Journal of nanobiotechnology · 2024Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging.Chemical & biomedical imaging · 2024Article
- Rapid and Label-Free Histopathology of Oral Lesions Using Deep Learning Applied to Optical and Infrared Spectroscopic Imaging Data.Journal of personalized medicine · 2024Article
- α-Synuclein emulsifies TDP-43 prion-like domain-RNA liquid droplets to promote heterotypic amyloid fibrils.Communications biology · 2023Article
- Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging.Nature communications · 2023Article
- High-Fidelity Micro- and Nano-Scale Infrared Spectroscopic Imaging.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel.Science advances · 2023Article
- Toward the Next Frontiers of Vibrational Bioimaging.Chemical & biomedical imaging · 2023Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Nearfield spectroscopic imaging techniques can be a powerful tool to map both cellular ultrastructure and molecular composition simultaneously but are currently limited in measurement capability. Resonance enhanced (RE) atomic force microscopy infrared (AFM-IR) spectroscopic imaging offers high-sensitivity measurements, for example, but probe-sample mechanical coupling, nonmolecular optical gradient forces, and noise overwhelm recorded chemical signals. Here, we analyze the key factors limiting AFM-IR measurements and propose an instrument design that enables high-sensitivity nanoscale IR imaging by combining null-deflection measurements with RE sensitivity. Our developed null-deflection scanning probe IR (NDIR) spectroscopic imaging provides ∼24× improvement in signal-to-noise ratio (SNR) compared with the state of the art, enables optimal signal recording by combining cantilever resonance with maximum laser power, and reduces background nonmolecular signals for improved analytical accuracy. We demonstrate the use of these properties for high-sensitivity, hyperspectral imaging of chemical domains in 100-nm-thick sections of cellular acini of a prototypical cancer model cell line, MCF-10A. NDIR chemical imaging enables facile recording of label-free, chemically accurate, high-SNR vibrational spectroscopic data from nanoscale domains, paving the path for routine studies of biomedical, forensic, and materials samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.