Evidence map›Paper›PMID 36374770›Full record

Trial reportEBioMedicine2022

Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome.

Andras T Meszaros, Julia Hofmann, Madita L Buch, Benno Cardini, Theresia Dunzendorfer-Matt, Florian Nardin, Michael J Blumer, Margot Fodor, Martin Hermann, Bettina Zelger and 12 more

Open access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in EBioMedicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
7.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.

  1. Donor-Related Risk Factors for Normothermic Machine Perfusion in Liver Transplantation: A Meta-Analysis.Liver international : official journal of the International Association for the Study of the Liver · 2025
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  12. Beyond preservation: future machine perfusion for liver assessment and repair.Nature reviews. Gastroenterology & hepatology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 2 institutions in 1 country.

Andras T MeszarosDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Julia HofmannDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Madita L BuchDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Benno CardiniDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Theresia Dunzendorfer-MattInstitute of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
Florian NardinDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria; Department of Anatomy, Histology and Embryology, Division of Clinical and Functional Anatomy, Medical University of Innsbruck, Innsbruck, Austria.
Michael J BlumerDepartment of Anatomy, Histology and Embryology, Division of Clinical and Functional Anatomy, Medical University of Innsbruck, Innsbruck, Austria.
Margot FodorDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Martin HermannDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Bettina ZelgerInstitute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck, Innsbruck, Austria.
Giorgi OtarashviliDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Melanie SchartnerDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Annemarie WeissenbacherDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Rupert OberhuberDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Thomas ReschDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Jakob TroppmairDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Dietmar FnerDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Heinz ZollerDepartment of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria.
Herbert TilgDepartment of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria.
Erich GnaigerOroboros Instruments, Innsbruck, Austria.
Theresa HautzDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Stefan SchneebergerDepartment of Visceral, Transplant and Thoracic Surgery, organLife... Laboratory and Daniel Swarovski Research Laboratory, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria. Electronic address: stefan.schneeberger@i-med.ac.at.
Innsbruck Medical University · ATOroboros Instruments (Austria) · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundReliable biomarkers for organ quality assessment during normothermic machine perfusion (NMP) are desired. ATP (adenosine triphosphate) production by oxidative phosphorylation plays a crucial role in the bioenergetic homeostasis of the liver. Thus, detailed analysis of the aerobic mitochondrial performance may serve as predictive tool towards the outcome after liver transplantation.

methodsIn a prospective clinical trial, 50 livers were subjected to NMP (OrganOx Metra) for up to 24.ßh. Biopsy and perfusate samples were collected at the end of cold storage, at 1.ßh, 6.ßh, end of NMP, and 1.ßh after reperfusion. Mitochondrial function and integrity were characterized by high-resolution respirometry (HRR), AMP, ADP, ATP and glutamate dehydrogenase analysis and correlated with the clinical outcome (L-GrAFT score). Real-time confocal microscopy was performed to assess tissue viability. Structural damage was investigated by histology, immunohistochemistry and transmission electron microscopy.

findingsA considerable variability in tissue viability and mitochondrial respiration between individual livers at the end of cold storage was observed. During NMP, mitochondrial respiration with succinate and tissue viability remained stable. In the multivariate analysis of the 35 transplanted livers (15 were discarded), area under the curve (AUC) of LEAK respiration, cytochrome c control efficiency (mitochondrial outer membrane damage), and efficacy of the mitochondrial ATP production during the first 6.ßh of NMP correlated with L-GrAFT. INTERPRETATIONS: Bioenergetic competence during NMP plays a pivotal role in addition to tissue injury markers. The AUC for markers of outer mitochondrial membrane damage, ATP synthesis efficiency and dissipative respiration (LEAK) predict the clinical outcome upon liver transplantation.

fundingThis study was funded by a Grant from the In Memoriam Dr. Gabriel Salzner Stiftung awarded to SS and the Tiroler Wissenschaftsfond granted to TH.

Indexed as

Cold IschemiaOrgan PreservationAdenosine TriphosphateHumansLiverMitochondriaPerfusionProspective StudiesRespirationAdenosine TriphosphateHigh-resolution respirometryLiverMitochondriaNormothermic machine perfusionTransplantation

Identifiers

PMID36374770
PMCPMC9626552
OpenAlexW4308158145

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.