Evidence map›Paper›PMID 36374463›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.

Danielle J Dauphars, Glendon Wu, Craig H Bassing, Michael S Krangel

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Danielle J Dauphars *Department of Immunology, Duke University School of Medicine, Durham, NC, USA.
Glendon Wu *Immunology Graduate Group, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
Craig H BassingImmunology Graduate Group, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA. bassing@email.chop.edu.
Michael S KrangelDepartment of Immunology, Duke University School of Medicine, Durham, NC, USA. krang001@mc.duke.edu.
Duke University · USChildren's Hospital of Philadelphia · USUniversity of Pennsylvania · US

Funding

Basic Immunology Training ProgramT32AI052077 · NIAID · DUKE UNIVERSITY · PI KRANGEL, MICHAEL S · 2002 to 2024
$4.2M
Immune System Development and RegulationT32AI055428 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ALLMAN, DAVID M, OLIVER, PAULA MARIA · 2003 to 2025
$4.2M
Topological Control of Antigen Receptor Loci during Lymphocyte DevelopmentR01AI130231 · NIAID · CHILDREN'S HOSP OF PHILADELPHIA · PI BASSING, CRAIG H, OLTZ, EUGENE M · 2017 to 2021
$3.8M
Chromatin regulation of TCR locus V(D)J recombinationR35GM136284 · NIGMS · DUKE UNIVERSITY · PI KRANGEL, MICHAEL S · 2020 to 2024
$2.4M
DNA Double Strand Break Mediated Feedback Inhibition of V(D)J RecombinationR01AI112621 · NIAID · CHILDREN'S HOSP OF PHILADELPHIA · PI BASSING, CRAIG H · 2015 to 2019
$1.9M
NIAID NIH HHS R01 AI112621NIAID NIH HHS R01 AI130231NIAID NIH HHS T32 AI052077NIAID NIH HHS T32 AI055428NIGMS NIH HHS R35 GM136284
6 · The paper itself

Abstract

Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice.

Indexed as

Gene RearrangementReceptors, Antigen, T-Cell, alpha-betaAnimalsMicePolymerase Chain ReactionReceptors, Antigen, T-Cell, alpha-betaT cell receptor (Tcr) genesT cell receptor (TCR) proteinsThymocytesVariable (V), diversity (D), and joining (J) gene segmentsVDJ recombinationαβ T cells

Identifiers

PMID36374463
OpenAlexW4309002027

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.