Evidence map›Paper›PMID 36374458›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Large-Scale Isolation of Mouse Thymic Epithelial Cells.

Izumi Ohigashi, Mami Matsuda-Lennikov, Yousuke Takahama

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 2 countries.

Izumi OhigashiDivision of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima, Japan.
Mami Matsuda-LennikovExperimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Yousuke TakahamaExperimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. yousuke.takahama@nih.gov.
National Cancer Institute · USNational Institutes of Health · USTokushima University · JP

Funding

Thymus biologyZIABC011806 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI TAKAHAMA, YOUSUKE · 2018 to 2025
$13.5M
Intramural NIH HHS ZIA BC011806
6 · The paper itself

Abstract

The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.

Indexed as

ProteomicsThymus GlandAnimalsCell DifferentiationEpithelial CellsMiceT-LymphocytesCell sortingcTECmTECProteomeThymic epithelial cell

Identifiers

PMID36374458
PMCPMC10280300
OpenAlexW4309013908

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.