Evidence map›Paper›PMID 36374452›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Principles of Advanced Flow Cytometry: A Practical Guide.

Shafiuddin Siddiqui, Ferenc Livák

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Spectral Flow Cytometry: The Current State and Future of the Technology.International journal of molecular sciences · 2025
    Review
  12. Article
  13. Article
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Shafiuddin SiddiquiCenter for Cancer Research, Laboratory of Genome Integrity, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Ferenc LivákCenter for Cancer Research, Laboratory of Genome Integrity, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. ferenc.livak@nih.gov.

Funding

CCR, LGI, Flow Cytometry Core FacilityZICBC011428 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI LIVAK, FERENC · 2012 to 2025
$17.3M
Intramural NIH HHS ZIC BC011428
6 · The paper itself

Abstract

Recent advances have revolutionized the oldest high-throughput single-cell analytical tool, flow cytometry. Fluorescent analyzers and sorters with up to seven lasers and the potential to detect up to 50 parameters are changing the way flow cytometry is used, but old school practices which are inadequate for new technologies remain alive. This chapter summarizes recent advances, explains the most salient new features and offers a step-by-step guide to develop and successfully execute high-dimensional fluorescent flow cytometry experiments.

Indexed as

LasersLightFlow CytometryAntibodyHigh-dimensional flow cytometryImmunofluorescenceImmunologySingle-cell analysis

Identifiers

PMID36374452
PMCPMC10802916

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.