ArticleMethods in molecular biology (Clifton, N.J.)2023
Producing Biologics with Defined N-Glycosylation in Plants.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Characterization of a plant-derived monoclonal antibody targeting extracellular enveloped virions of Monkeypox virus.Frontiers in plant science · 2024Article
- A Dual-Approach Strategy to Optimize the Safety and Efficacy of Anti-Zika Virus Monoclonal Antibody Therapeutics.Viruses · 2023Article
- A novel plant-made monoclonal antibody enhances the synergetic potency of an antibody cocktail against the SARS-CoV-2 Omicron variant.Plant biotechnology journal · 2023Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proper glycosylation of glycoproteins is important for their structure and function. This is an especially important consideration when choosing a platform to express recombinant glycoproteins destined for therapeutic use. Chinese hamster ovary (CHO) cells have been the choice expression platform for their ability to produce recombinant glycoproteins with glycosylation profiles similar to those observed in humans. However, consistency with glycosylation has been noted as problematic, and sialylation, an important modification in human glycoproteins, has not been achieved to an acceptable degree in CHO cells. Plant biotechnology and glycoengineering has now made it possible to produce therapeutic recombinant glycoproteins in plants with glycosylation profiles observed in humans, including sialylation. Furthermore, the glycosylation profiles of recombinant therapeutic glycoproteins produced in plants are homogenous and consistently reproducible. Here, entirely via transient expression, two therapeutic monoclonal antibodies are produced in glycoengineered Nicotiana benthamiana plants that carry human glycosylation profiles including sialylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.