ArticlePlant biotechnology journal2023
Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- An overview of recent advances on wheat homologous and homoeologous recombination.Journal of experimental botany · 2026Review
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Genetic manipulation of the genes for clonal seeds results in sterility in cotton.BMC plant biology · 2024Article
- HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania.Nature communications · 2023Article
- Comprehensive dissection of meiotic DNA double-strand breaks and crossovers in cucumber.Plant physiology · 2023Article
- Fighting wheat powdery mildew: from genes to fields.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Engineering apomixis in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- Molecular Cytological Analysis and Specific Marker Development in Wheat-International journal of molecular sciences · 2023Article
- ASYNAPSIS 1 ensures crossover fidelity in polyploid wheat by promoting homologous recombination and suppressing non-homologous recombination.Frontiers in plant science · 2023Article
- Article
- Green revolution to genome revolution: driving better resilient crops against environmental instability.Frontiers in genetics · 2023Review
- Crossover interference mechanism: New lessons from plants.Frontiers in cell and developmental biology · 2023Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Increasing crop yields through plant breeding is time consuming and laborious, with the generation of novel combinations of alleles being limited by chromosomal linkage blocks and linkage-drag. Meiotic recombination is essential to create novel genetic variation via the reshuffling of parental alleles. The exchange of genetic information between homologous chromosomes occurs at crossover (CO) sites but CO frequency is often low and unevenly distributed. This bias creates the problem of linkage-drag in recombination 'cold' regions, where undesirable variation remains linked to useful traits. In plants, programmed meiosis-specific DNA double-strand breaks, catalysed by the SPO11 complex, initiate the recombination pathway, although only ~5% result in the formation of COs. To study the role of SPO11-1 in wheat meiosis, and as a prelude to manipulation, we used CRISPR/Cas9 to generate edits in all three SPO11-1 homoeologues of hexaploid wheat. Characterization of progeny lines shows plants deficient in all six SPO11-1 copies fail to undergo chromosome synapsis, lack COs and are sterile. In contrast, lines carrying a single copy of any one of the three wild-type homoeologues are phenotypically indistinguishable from unedited plants both in terms of vegetative growth and fertility. However, cytogenetic analysis of the edited plants suggests that homoeologues differ in their ability to generate COs and in the dynamics of synapsis. In addition, we show that the transformation of wheat mutants carrying six edited copies of SPO11-1 with the TaSPO11-1B gene, restores synapsis, CO formation, and fertility and hence opens a route to modifying recombination in this agronomically important crop.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.