ArticleCommunications biology2022
Biosynthesis of cannabinoid precursor olivetolic acid in genetically engineered Yarrowia lipolytica.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Toward Complex Polyketide Biosynthesis inJournal of microbiology and biotechnology · 2026Review
- Metabolic engineering of Synechocystis sp. PCC 6803 for olivetolic acid production.Microbial cell factories · 2026Article
- Recent innovations in cannabinoid chemistry, biology, and biosynthesis.Current opinion in biotechnology · 2025Review
- Article
- Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl-CoA Pathway at High Titer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- High light intensity enhances cannabinoid biosynthesis through concerted gene expression in hemp (Frontiers in plant science · 2025Article
- Identification and Optimization of more Efficient Olivetolic Acid Synthases.Cannabis and cannabinoid research · 2024Article
- Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD).ACS synthetic biology · 2024Review
- Bioengineering of the Marine DiatomInternational journal of molecular sciences · 2023Article
- Article
- Biotechnological Fungal Platforms for the Production of Biosynthetic Cannabinoids.Journal of fungi (Basel, Switzerland) · 2023Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Engineering microbes to produce plant-derived natural products provides an alternate solution to obtain bioactive products. Here we report a systematic approach to sequentially identify the rate-limiting steps and improve the biosynthesis of the cannabinoid precursor olivetolic acid (OLA) in Yarrowia lipolytica. We find that Pseudomonas sp LvaE encoding a short-chain acyl-CoA synthetase can efficiently convert hexanoic acid to hexanoyl-CoA. The co-expression of the acetyl-CoA carboxylase, the pyruvate dehydrogenase bypass, the NADPH-generating malic enzyme, as well as the activation of peroxisomal β-oxidation pathway and ATP export pathway are effective strategies to redirect carbon flux toward OLA synthesis. Implementation of these strategies led to an 83-fold increase in OLA titer, reaching 9.18 mg/L of OLA in shake flask culture. This work may serve as a baseline for engineering cannabinoids biosynthesis in oleaginous yeast species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.