Evidence map›Paper›PMID 36371461›Full record

ArticleCommunications biology2022

Microfluidic live tracking and transcriptomics of cancer-immune cell doublets link intercellular proximity and gene regulation.

Bianca C T Flores, Smriti Chawla, Ning Ma, Chad Sanada, Praveen Kumar Kujur, Rudy Yeung, Margot B Bellon, Kyle Hukari, Brian Fowler, Mark Lynch and 4 more

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07779317 (Superior Venous Access), which is not on this map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07779317 narecruitingnot on this mapstarted 2026, after this paper: background citation

Superior Venous Access: Single Lumen Midline Catheter vs Ultrasound Guided Peripheral IV as Performed by Bedside Emergency Department Registered Nurses, A Randomized Clinical Trial

TypeinterventionalSponsorAlbany Medical CollegeRan2026 to 2027Enrolled292ConditionsDifficult Vein Access, Emergency Department Patients, Nurses Role, Midline CatheterArmsUltrasound-Guided Single-Lumen Midline Catheter, Ultrasound-Guided Long Peripheral Intravenous Catheter
3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Bianca C T Flores *Department of Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.ORCID 0000-0002-6490-6309
Smriti Chawla *Department of Computational Biology, Indraprastha Institute of Information Technology, New Delhi, 110020, India.
Ning Ma *Department of Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Chad SanadaNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA.
Praveen Kumar KujurDepartment of Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Rudy YeungNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA.
Margot B BellonDepartment of Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.ORCID 0000-0002-0040-2523
Kyle HukariNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA.
Brian FowlerNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA.
Mark LynchNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA.
Ludmilla T D ChinenCirculating Tumor Cells Group, A.C.Camargo Cancer Center, São Paulo, SP, 01508-010, Brazil.
Naveen RamalingamNew Technologies Group, Fluidigm Corporation, South San Francisco, CA, 94080, USA. naveen.ramalingam@fluidigm.com.
Debarka SenguptaDepartment of Computational Biology, Indraprastha Institute of Information Technology, New Delhi, 110020, India. debarka@iiitd.ac.in.ORCID 0000-0002-6353-5411
Stefanie S JeffreyDepartment of Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA. ssj@stanford.edu.ORCID 0000-0003-4478-2764

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-cell communication and physical interactions play a vital role in cancer initiation, homeostasis, progression, and immune response. Here, we report a system that combines live capture of different cell types, co-incubation, time-lapse imaging, and gene expression profiling of doublets using a microfluidic integrated fluidic circuit that enables measurement of physical distances between cells and the associated transcriptional profiles due to cell-cell interactions. We track the temporal variations in natural killer-triple-negative breast cancer cell distances and compare them with terminal cellular transcriptome profiles. The results show the time-bound activities of regulatory modules and allude to the existence of transcriptional memory. Our experimental and bioinformatic approaches serve as a proof of concept for interrogating live-cell interactions at doublet resolution. Together, our findings highlight the use of our approach across different cancers and cell types.

Indexed as

TranscriptomeTriple Negative Breast NeoplasmsGene Expression ProfilingGene Expression RegulationHumansMicrofluidics

Identifiers

PMID36371461
PMCPMC9653407

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.