Evidence map›Paper›PMID 36370357›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo.

Kazumi Kawata, Satoshi Kubota

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Kazumi KawataDepartment of Anatomy and Cell Biology, University of Yamanashi Interdisciplinary Graduate School of Medicine and Engineering, Chuo, Yamanashi, Japan. ph092dix@okayama-u.ac.jp.
Satoshi KubotaDepartment of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Kita-ku, Okayama, Japan.
Okayama University · JPUniversity of Yamanashi · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dental pulp cells (DPCs) differentiate into odontoblasts. To observe odontoblastic differentiation, the detection of dentinogenesis-specific molecules such as dentin sialophosphoprotein (DSPP) and the measurement of alkaline phosphatase (ALP) activity are reliable approaches. CCN family member 2 (CCN2) has been proposed as a marker for dentinogenesis. Our recent study revealed that the expression levels of Ccn4, Ccn5, and Ccn6 were changed in accordance with odontoblastic differentiation. Therefore, Ccn4, Ccn5, and Ccn6, as well as Ccn2, could serve as a comprehensive set of markers for dentinogenesis. Here, we describe a method of measuring the Ccns expression levels in differentiating rat DPCs.

Indexed as

Alkaline PhosphataseOdontoblastsAnimalsCell DifferentiationCells, CulturedDental PulpExtracellular Matrix ProteinsGene ExpressionPhosphoproteinsRatsAlkaline PhosphataseExtracellular Matrix ProteinsPhosphoproteinsDental pulp cellsDentinogenesisOdontoblastsRat teethReal-time PCRWestern blot

Identifiers

PMID36370357
OpenAlexW4309081510

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.