Evidence map›Paper›PMID 36370286›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status.

Marie Vajrychova, Barbora Salovska, Kristyna Pimkova, Ivo Fabrik, Zdenek Hodny

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marie VajrychovaBiomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic. marie.vajrychova@fnhk.cz.
Barbora SalovskaDepartment of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Science, Prague, Czech Republic.
Kristyna PimkovaBiomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Ivo FabrikBiomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Zdenek HodnyDepartment of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Science, Prague, Czech Republic.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) and iodoacetyl tandem mass tag (iodoTMT) are well-implemented mass spectrometry-based approaches for quantification of proteins and for site-mapping of cysteine modification. We describe here a combination of SILAC and iodoTMT to assess ongoing changes in the global proteome and cysteine modification levels using liquid chromatography separation coupled with high-resolution mass spectrometry (LC-MS/MS).

Indexed as

ProteomeProteomicsChromatography, LiquidCysteineIsotope LabelingOxidation-ReductionTandem Mass SpectrometryCysteineProteomeCysteineGlobal proteomeIodoTMTLiquid chromatographyMass spectrometryRedoxSILAC

Identifiers

PMID36370286

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.