Evidence map›Paper›PMID 36370280›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos.

Alistair Langlands, Hamze Beati, H- Arno J Müller

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alistair LanglandsNational Phenotypic Screening Centre, School of Life Sciences, University of Dundee, Dundee, UK.
Hamze BeatiDevelopmental Genetics Group, Institute for Biology, University of Kassel, Kassel, Germany.
H- Arno J MüllerDevelopmental Genetics Group, Institute for Biology, University of Kassel, Kassel, Germany. h.a.muller@uni-kassel.de.

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
Medical Research Council KO18531/1NIH HHS P40 OD018537Wellcome Trust
6 · The paper itself

Abstract

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Indexed as

Drosophila melanogasterProteomicsAnimalsDrosophilaIsotope LabelingProtein Processing, Post-TranslationalDevelopmentDrosophilaEmbryoPosttranslational modificationProteomicsSILAC

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.