Evidence map›Paper›PMID 36370267›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.

Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, Juraj Gregan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Dorothea AnratherMass Spectrometry Facility, Max Perutz Labs, Vienna BioCenter (VBC), Vienna, Austria. dorothea.anrather@univie.ac.at.
Silvia Bagelova PolakovaInstitute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia. silvia.bagelova@savba.sk.
Lubos CipakCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia. lubos.cipak@savba.sk.
Juraj GreganAdvanced Microscopy Facility, VBCF and Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Vienna, Austria. juraj.gregan@univie.ac.at.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) provides a powerful tool to quantify proteins and posttranslational modifications. Here we describe how to apply SILAC for protein identification and quantification in synchronous meiotic cultures induced by inactivation of the Pat1 kinase in the fission yeast Schizosaccharomyces pombe.

Indexed as

SchizosaccharomycesSchizosaccharomyces pombe ProteinsMeiosisProteinsProteomicsProteinsSchizosaccharomyces pombe ProteinsMass SpectrometryMeiosisPat1ProteomeSchizosaccharomyces pombeSILACYeast

Identifiers

PMID36370267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.