Evidence map›Paper›PMID 36369591›Full record

ArticleAnalytical and bioanalytical chemistry2023

Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry.

Karen Duong, Simin Maleknia, David Clases, Andrew Minett, Matthew P Padula, Philip A Doble, Raquel Gonzalez de Vega

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Karen DuongThe Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.
Simin MalekniaSchool of Mathematical and Physical Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Australia.
David ClasesThe Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.
Andrew MinettePrep Pty Ltd, Oakleigh, Victoria, 3166, Australia.
Matthew P PadulaSchool of Life Sciences and Proteomics Core Facility, Faculty of Science, University of Technology Sydney, Ultimo, Australia.
Philip A DobleThe Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia.
Raquel Gonzalez de VegaThe Atomic Medicine Initiative, University of Technology Sydney, Ultimo, Australia. raquel.gonzalez-de-vega@uni-graz.at.
University of Technology Sydney · AUUniversity of Graz · ATIP Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This work describes a novel automated and rapid method for bottom-up proteomics combining protein isolation with a micro-immobilised enzyme reactor (IMER). Crosslinking chemistry based on 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling was exploited to immobilise trypsin and antibodies onto customisable silica particles coated with carboxymethylated dextran (CMD). This novel silica-CMD solid-phase extraction material was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), conductometric titrations and enzymatic colorimetric assays. Micro-solid-phase extraction (μSPE) cartridges equipped with the modified CMD material were employed and integrated into an automated and repeatable workflow using a sample preparation workstation to achieve rapid and repeatable protein isolation and pre-concentration, followed by tryptic digestion producing peptide fragments that were identified by liquid chromatography mass spectrometry (LC-MS).

Indexed as

Enzymes, ImmobilizedProteinsDigestionMass SpectrometrySilicon DioxideSolid Phase ExtractionTrypsinEnzymes, ImmobilizedProteinsSilicon DioxideTrypsinIMERImmobilised enzymeImmunosorbentMass spectrometryProtein analysisμSPE

Identifiers

PMID36369591
PMCPMC10328895
OpenAlexW4308834538

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.