Evidence map›Paper›PMID 36369276›Full record

ArticleCommunications biology2022

High-throughput determination of dry mass of single bacterial cells by ultrathin membrane resonators.

Adrián Sanz-Jiménez, Oscar Malvar, Jose J Ruz, Sergio García-López, Priscila M Kosaka, Eduardo Gil-Santos, Álvaro Cano, Dimitris Papanastasiou, Diamantis Kounadis, Jesús Mingorance and 3 more

Abstract read
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Adrián Sanz-JiménezInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.
Oscar MalvarInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0001-9860-0634
Jose J RuzInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0003-4604-7042
Sergio García-LópezInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.
Priscila M KosakaInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0003-4242-7064
Eduardo Gil-SantosInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0002-7100-934X
Álvaro CanoInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.
Dimitris PapanastasiouFasmatech Science & Technology, Lefkippos TESPA, Demokritos NCSR, Patriarchou Gregoriou & Neapoleos, 15341, Athens, Greece.
Diamantis KounadisFasmatech Science & Technology, Lefkippos TESPA, Demokritos NCSR, Patriarchou Gregoriou & Neapoleos, 15341, Athens, Greece.
Jesús MingoranceHospital Universitario La Paz, IdiPAZ, 28046, Madrid, Spain.ORCID 0000-0001-6173-5711
Álvaro San PauloInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0001-9325-8892
Montserrat CallejaInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.ORCID 0000-0003-2414-5725
Javier TamayoInstituto de Micro y Nanotecnología, IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain. javier.tamayo@csic.es.ORCID 0000-0002-5280-7215

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

How bacteria are able to maintain their size remains an open question. Techniques that can measure the biomass (dry mass) of single cells with high precision and high-throughput are demanded to elucidate this question. Here, we present a technological approach that combines the transport, guiding and focusing of individual bacteria from solution to the surface of an ultrathin silicon nitride membrane resonator in vacuum. The resonance frequencies of the membrane undergo abrupt variations at the instants where single cells land on the membrane surface. The resonator design displays a quasi-symmetric rectangular shape with an extraordinary capture area of 0.14 mm

Indexed as

Staphylococcus epidermidisVibration

Identifiers

PMID36369276
PMCPMC9651879

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.